外源性p53基因增加卵巢癌细胞对顺铂的敏感性

R737.31; 目的探讨外源性p53基因转染对人卵巢癌细胞株的化疗敏感性的影响. 方法用脂质体介导的转染技术,将人野生型p53基因的真核表达载体导入不表达p53的卵巢癌SKOV-3细胞中,经G418筛选,Northern blot及Western blot鉴定后,观察其对顺铂作用后的SKOV-3细胞的集落形成及凋亡的影响.结果外源性p53基因在转染细胞中有效表达,并增强了顺铂对SKOV-3细胞集落形成的抑制作用及促进了顺铂诱导的细胞凋亡.结论外源性p53基因能增加卵巢癌细胞对顺铂的敏感性,两者联合作用能更大程度地杀灭肿瘤细胞....

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Published in肿瘤防治研究 Vol. 29; no. 1; pp. 35 - 36
Main Authors 关婷, 崔满华, 李守柔
Format Journal Article
LanguageChinese
Published 广州军区广州总医院妇产科,510010%白求恩医科大学第二临床学院妇产科 2002
Subjects
卵巢癌
顺铂
化疗敏感性
p53
Online AccessGet full text
ISSN1000-8578
DOI10.3971/j.issn.1000-8578.2002.01.014

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Abstract R737.31; 目的探讨外源性p53基因转染对人卵巢癌细胞株的化疗敏感性的影响. 方法用脂质体介导的转染技术,将人野生型p53基因的真核表达载体导入不表达p53的卵巢癌SKOV-3细胞中,经G418筛选,Northern blot及Western blot鉴定后,观察其对顺铂作用后的SKOV-3细胞的集落形成及凋亡的影响.结果外源性p53基因在转染细胞中有效表达,并增强了顺铂对SKOV-3细胞集落形成的抑制作用及促进了顺铂诱导的细胞凋亡.结论外源性p53基因能增加卵巢癌细胞对顺铂的敏感性,两者联合作用能更大程度地杀灭肿瘤细胞.
AbstractList R737.31; 目的探讨外源性p53基因转染对人卵巢癌细胞株的化疗敏感性的影响. 方法用脂质体介导的转染技术,将人野生型p53基因的真核表达载体导入不表达p53的卵巢癌SKOV-3细胞中,经G418筛选,Northern blot及Western blot鉴定后,观察其对顺铂作用后的SKOV-3细胞的集落形成及凋亡的影响.结果外源性p53基因在转染细胞中有效表达,并增强了顺铂对SKOV-3细胞集落形成的抑制作用及促进了顺铂诱导的细胞凋亡.结论外源性p53基因能增加卵巢癌细胞对顺铂的敏感性,两者联合作用能更大程度地杀灭肿瘤细胞.
Author 李守柔
崔满华
关婷
AuthorAffiliation 广州军区广州总医院妇产科,510010%白求恩医科大学第二临床学院妇产科
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DocumentTitle_FL Enhanced sensitivity of human ovarian cancer cells to cisplatin by exogenous p53 gene
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顺铂
化疗敏感性
p53
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Snippet R737.31; 目的探讨外源性p53基因转染对人卵巢癌细胞株的化疗敏感性的影响. 方法用脂质体介导的转染技术,将人野生型p53基因的真核表达载体导入不表达p53的卵巢癌SKOV-3细胞中,经G418筛选,Northern blot及Western...
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Title 外源性p53基因增加卵巢癌细胞对顺铂的敏感性
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