Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

• Published in: Veterinary and animal science Vol. 16; p. 100241
• Main Authors: Namula, Zhao, Wittayarat, Manita, Do, Lanh Thi Kim, Van Nguyen, Thanh, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Tanihara, Fuminori, Otoi, Takeshige
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.06.2022; Elsevier
• Subjects: CRISPR/Cas9; Electroporation; Gene knockout; Permeability; Pig; Electroporation; Permeability; CRISPR/Cas9; Gene knockout; Pig

•Mosaicism is a serious problem for genome editing during embryogenesis.•We hypothesized that genome-editing before in vitro fertilization can increase its efficiency.•We introduced CRISPR/Cas9 system into oocytes during in vitro maturation using electroporation.•Gene editing efficiency in matured o...