Epigenetic transcriptional silencing and 5-azacytidine-mediated reactivation of a complex transgene in rice

• Published in: Plant physiology (Bethesda) Vol. 115; no. 2; pp. 361 - 373
• Main Authors: Kumpatla, S.P, Teng, W, Buchholz, W.G, Hall, T.C
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.10.1997
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; ANTIBIOTICOS; ANTIBIOTICS; ANTIBIOTIQUE; Azacitidine - pharmacology; Bacterial Proteins - genetics; Bacterial Toxins; BILANAFOS; Biological and medical sciences; BTT CRYIIIA TRANSGENES; CARTE GENETIQUE; CITIDINA; Classical genetics, quantitative genetics, hybrids; CODE GENETIQUE; CODIGO GENETICO; CYTIDINE; Cytosine - metabolism; DERIVATIVES; DNA; DNA METHYLATION; DNA probes; Drug Resistance - genetics; Endotoxins - genetics; ENZYME INHIBITORS; ENZYMIC ACTIVITY; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene Expression Regulation, Plant - drug effects; Gene Rearrangement; Gene Regulation and Molecular Genetics; Gene silencing; GENE TRANSFER; GENETIC CODE; GENETIC INHERITANCE; GENETIC MAPS; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; Genomics; Hemolysin Proteins; HERBICIDAS; HERBICIDE; HERBICIDES; Herbicides - pharmacology; HEREDITE; HERENCIA GENETICA; INHIBICION; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; INHIBITION; INSECTICIDAL PROPERTIES; INSECTICIDAS; INSECTICIDE; INSECTICIDES; LOCI; LOCUS; MAPAS GENETICOS; Methylation; Models, Genetic; NUCLEASAS; NUCLEASE; NUCLEASES; Organophosphorus Compounds - pharmacology; Oryza - genetics; ORYZA SATIVA; PLANT PROTEINS; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants; Plants, Genetically Modified; Promoter regions; PROMOTERS; PROTEINAS; PROTEINE; PROTEINS; Pteridophyta, spermatophyta; RESISTANCE AUX PRODUITS CHIMIQUES; RESISTANCE TO CHEMICALS; RESISTENCIA A PRODUCTOS QUIMICOS; Rice; SEGREGACION; SEGREGATION; TRANSCRIPCION; TRANSCRIPTION; Transcription, Genetic; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; Transformation, Genetic; Transgenes; TRANSGENIC PLANTS; Vegetals; Monocotyledones; Reactivation; Genetic inheritance; Gene expression; Cereal crop; Oryza sativa; Gene silencing; Regulation(control); Gramineae; Azacitidine; Angiospermae; Epigenetics; Spermatophyta; Transgenic plant; Methylation

Despite a growing number of reports indicating non-Mendelian inheritance of transgene expression in monocots, no detailed description of the structure and stability of the transgene exists for transformants generated by direct DNA-transfer techniques, making the cause for these observations difficul...