Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias

• Published in: Nature biotechnology Vol. 36; no. 2; pp. 190 - 195
• Main Authors: Karst, Søren M, Dueholm, Morten S, McIlroy, Simon J, Kirkegaard, Rasmus H, Nielsen, Per H, Albertsen, Mads
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.02.2018; Nature Publishing Group
• Subjects: 45/23; 45/77; 45/90; 631/114/1386; 631/326/2565/2134; 631/326/2565/855; 704/158/2452; 704/158/855; Agriculture; Archaea; Archaea - classification; Archaea - genetics; Bacteria - classification; Bacteria - genetics; Bias; Biodiversity; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Cattle; Chimerism; Ecosystems; Eukaryota - classification; Eukaryota - genetics; Eukaryotes; Gene sequencing; Genetic aspects; High-Throughput Nucleotide Sequencing; letter; Life Sciences; Metagenome - genetics; Methods; Microbial colonies; Microorganisms; Phylogeny; Polyadenine; Protozoa; Reverse transcription; Ribonucleic acid; RNA; RNA sequencing; RNA, Ribosomal, 16S - classification; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 18S - genetics; rRNA 16S; rRNA 18S

More of the diversity present in any microbiome is revealed by a method to sequence 16S rRNA that avoids primer bias. Small subunit ribosomal RNA (SSU rRNA) genes, 16S in bacteria and 18S in eukaryotes, have been the standard phylogenetic markers used to characterize microbial diversity and evolutio...