The serine protease inhibitor camostat inhibits influenza virus replication and cytokine production in primary cultures of human tracheal epithelial cells

• Published in: Pulmonary pharmacology & therapeutics Vol. 33; pp. 66 - 74
• Main Authors: Yamaya, Mutsuo, Shimotai, Yoshitaka, Hatachi, Yukimasa, Lusamba Kalonji, Nadine, Tando, Yukiko, Kitajima, Yasuo, Matsuo, Kaori, Kubo, Hiroshi, Nagatomi, Ryoichi, Hongo, Seiji, Homma, Morio, Nishimura, Hidekazu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2015
• Subjects: Aged; Airway epithelial cell; Animals; Aprotinin - pharmacology; Camostat; Cell culture; Cells, Cultured; Dogs; Epithelial Cells - drug effects; Epithelial Cells - virology; Female; Gabexate - analogs & derivatives; Gabexate - pharmacology; Humans; Influenza; Influenza A Virus, H1N1 Subtype - isolation & purification; Influenza A Virus, H3N2 Subtype - isolation & purification; Influenza, Human - drug therapy; Influenza, Human - virology; Interleukin; Madin Darby Canine Kidney Cells; Male; Medical Education; Middle Aged; Pulmonary/Respiratory; RNA, Viral - metabolism; Serine protease; Serine Proteinase Inhibitors - pharmacology; Trachea - cytology; Trachea - virology; Virus Replication - drug effects; Cell culture; RT; AEBSF; Interleukin; CLEIA; SARS-CoV; Camostat; TNF; LPS; IFN; DMEM; LDH; DF-12; ANOVA; MDCK; PBS; Airway epithelial cell; TMPRSS; IL; MOI; F-12; USG; PVDF; TCID; PBS-T; SDS-PAGE; MEM; Influenza; KIU; Serine protease; HA; HAT; COPD; ELISA; phosphate buffered saline with Tween ® 20; hemagglutinin; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 4-(2-aminoethyl)-benzenesulfonyl fluoride; Ultroser G; mixture of Dulbecco's modified Eagle's medium-Ham's F-12 medium; polyvinylidene difluoride; Kallikrein Inhibitor Unit; lactate dehydrogenase; Dulbecco's modified Eagle's medium; severe acute respiratory syndrome coronavirus; human trypsin-like protease; lipopolysaccharide; Eagle's minimum essential medium; room temperature; Ham's F-12 medium; multiplicity of infection; analysis of variance; interferon; chemiluminescent enzyme immunoassay; tumor necrosis factor; phosphate buffered saline; enzyme-linked immunosorbent assay; Madin Darby Canine Kidney; tissue culture infective dose; transmembrane protease serine S1 member; chronic obstructive pulmonary disease

Serine proteases act through the proteolytic cleavage of the hemagglutinin (HA) of influenza viruses for the entry of influenza virus into cells, resulting in infection. However, the inhibitory effects of serine protease inhibitors on influenza virus infection of human airway epithelial cells, and o...