Facile quantitative diagnostic testing for neutralizing antibodies against Chikungunya virus

• Published in: BMC infectious diseases Vol. 24; no. 1; pp. 1076 - 8
• Main Authors: Lin, Hui-Chung, Chang, Shu-Fen, Su, Chien-Ling, Hu, Huai-Chin, Chiao, Der-Jiang, Hsu, Yu-Lin, Lu, Hsuan-ying, Lin, Chang-Chi, Shu, Pei-Yun, Kuo, Szu-Cheng
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 30.09.2024; BioMed Central; BMC
• Subjects: Animals; Antibiotics; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Neutralizing - immunology; Antibodies, Viral - blood; Antibodies, Viral - immunology; Assaying; Biosafety; Care and treatment; Case-Control Studies; Chikungunya fever; Chikungunya Fever - diagnosis; Chikungunya Fever - immunology; Chikungunya virus; Chikungunya virus - immunology; Correlation analysis; Correlation coefficient; Correlation coefficients; Curve fitting; Dengue fever; Diagnostics; Disease control; Encephalitis; Evaluation; Health aspects; Heat; Humans; Immune status; Infections; Luciferase; Medical research; Monoclonal antibodies; Neutralization; Neutralization Tests - methods; Neutralizing; Neutralizing antibodies; Patients; Penicillin; Physiological aspects; Sensitivity analysis; Sensitivity and Specificity; Surrogate virus neutralization test; Surveillance; Vector-borne diseases; Viral antibodies; Virus-like replicon particle; Viruses; Taiwan; United States > US; Malaysia; Diagnostics; Surveillance; Neutralizing antibodies; Chikungunya virus; Virus-like replicon particle; Surrogate virus neutralization test; Vaccine

Viral neutralization (NT) assays can be used to determine the immune status of patients or assess the potency of candidate vaccines or therapeutic monoclonal antibodies (mAbs). Focus reduction neutralization test (FRNT) is a conventional neutralization test (cVNT) with superior specificity for measu...