Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes

• Published in: BMC infectious diseases Vol. 24; no. 1; p. 164
• Main Authors: Dung, Tran Thi Ngoc, Phat, Voong Vinh, Vinh, Chau, Lan, Nguyen Phu Huong, Phuong, Nguyen Luong Nha, Ngan, Le Thi Quynh, Thwaites, Guy, Thwaites, Louise, Rabaa, Maia, Nguyen, Anh T K, Duy, Pham Thanh
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.02.2024; BioMed Central; BMC
• Subjects: Acinetobacter baumannii; Antibiotics; Antigens; Antimicrobial agents; Antimicrobial resistance; Assaying; Bacteria; Bacteria, Pathogenic; Bacterial pneumonia; Care and treatment; Causes of; Diagnosis; Drug resistance; Drug resistance in microorganisms; E coli; Escherichia coli; Genes; Genetic aspects; Health aspects; Identification and classification; Israel; Klebsiella; Klebsiella pneumoniae; Low income groups; Lower respiratory tract infections; Melting curve; Melting curve analysis; Molecular diagnostics; Morbidity; Mortality; Multiplexing; Pathogenic microorganisms; Pathogens; Phenotypes; Pneumonia; Polymerase chain reaction; Pseudomonas aeruginosa; Real time; Real-time PCR; Respiratory tract; Respiratory tract infection; Respiratory tract infections; Sensitivity; Sputum; Staphylococcal infections; Staphylococcus aureus; Streptococcus infections; Streptococcus pneumoniae; Target detection; Vietnam; Israel; United Kingdom > UK; United States > US; Lower respiratory tract infections; Melting curve analysis; Molecular diagnostics; Antimicrobial resistance; Real-time PCR

Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus are major bacterial causes of lower respiratory tract infections (LRTIs) globally, leading to substantial morbidity and mortality. The rapid increase of antimi...