Synthetic Biology Toolbox and Chassis Development in Bacillus subtilis

• Published in: Trends in biotechnology (Regular ed.) Vol. 37; no. 5; pp. 548 - 562
• Main Authors: Liu, Yanfeng, Liu, Long, Li, Jianghua, Du, Guocheng, Chen, Jian
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2019; Elsevier Limited
• Subjects: Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacillus subtilis - physiology; Binding sites; Biology; Biotechnology; Carbon; cell factories; Chassis; chassis cells; Chromosomes; E coli; Enzymes; Ethanol; gene editing; Gene expression; Gene loci; Gene sequencing; Genetic engineering; genome; Genome editing; Genome, Bacterial; Genomes; Gram-positive bacteria; Library collections; Lignocellulose; Metabolic Engineering; Metabolism; Microorganisms; Plasmids; Proteins; Synthetic biology; Synthetic Biology - methods; synthetic biology toolboxes; chassis cells; metabolic engineering; Bacillus subtilis; synthetic biology toolboxes; cell factories

Based on technical advances in the sequencing and synthesis of genetic components as well as the genome, significant progress has recently been made in developing synthetic biology toolboxes and chassis for the model Gram-positive bacterium Bacillus subtilis. In this review, we discuss recently deve...