From crystal structure to in silico epitope discovery in the Burkholderia pseudomallei flagellar hook‐associated protein FlgK

• Published in: The FEBS journal Vol. 282; no. 7; pp. 1319 - 1333
• Main Authors: Gourlay, Louise J, Thomas, Rachael J, Peri, Claudio, Conchillo‐Solé, Oscar, Ferrer‐Navarro, Mario, Nithichanon, Arnone, Vila, Jordi, Daura, Xavier, Lertmemongkolchai, Ganjana, Titball, Richard, Colombo, Giorgio, Bolognesi, Martino
• Format: Journal Article
• Language: English
• Published: England Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies 01.04.2015; Blackwell Publishing Ltd
• Subjects: Amino Acid Sequence; Animals; antibiotics; antibodies; Antibodies, Bacterial - blood; antigen; Antigens, Bacterial - chemistry; antiserum; Australia; bacterial motility; Bacterial Proteins - chemistry; Bacterial Proteins - immunology; Bacterial Proteins - physiology; Burkholderia pseudomallei; Burkholderia pseudomallei - immunology; Burkholderia pseudomallei; Cell Line; Computer Simulation; Crystal structure; Crystallography, X-Ray; cytotoxicity; engineering; epitope discovery; epitopes; Epitopes - chemistry; flagellar hook‐associated protein; flagellin; flagellum; Gram-negative bacteria; Humans; Immunoglobulins; macrophages; Macrophages - immunology; Macrophages - microbiology; melioidosis; Melioidosis - blood; Melioidosis - immunology; Melioidosis - microbiology; Mice; Models, Molecular; Molecular Sequence Data; pathogenesis; patients; prediction; protein subunits; Proteins; seroprevalence; South East Asia; structural vaccinology; Vaccines; virulence; South East Asia; Australia; flagellar hook-associated protein; antigen; epitope discovery; structural vaccinology; Burkholderia pseudomallei

Melioidosis, caused by the Gram‐negative bacterium Burkholderia pseudomallei, is a potentially fatal infection that is endemic in Southeast Asia and Northern Australia that is poorly controlled by antibiotics. Research efforts to identify antigenic components for a melioidosis vaccine have led to th...