Antibodies against human muscle enolase recognize a 45-kDa bacterial cell wall outer membrane enolase-like protein

• Published in: FEMS immunology and medical microbiology Vol. 45; no. 1; pp. 53 - 62
• Main Authors: Witkowska, Danuta, Pietkiewicz, Jadwiga, Szostko, Bernadeta, Danielewicz, Regina, Masłowski, Leszek, Gamian, Andrzej
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.07.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Affinity; Affinity chromatography; Animals; Antibodies; Antibodies - immunology; Arteriosclerosis; Atherosclerosis; Atherosclerosis - immunology; Bacteria; Bacterial Outer Membrane Proteins - immunology; Bacteriology; Biological and medical sciences; Cardiac muscle; Cell walls; Chromatography; Cross Reactions; Cross-reactivity; Cytoplasm; Damage detection; Dimers; Electrophoresis; Enolase; Enterobacteriaceae; Enterobacteriaceae - immunology; Epitopes; Epitopes - analysis; Fundamental and applied biological sciences. Psychology; Gel electrophoresis; Glycolysis; Gram-negative bacteria; Humans; Immunoblotting; Immunoglobulins; Isoforms; Klebsiella; Klebsiella pneumoniae; Mass spectrometry; Mass spectroscopy; Membrane proteins; Membranes; Microbiology; Miscellaneous; Muscle, Skeletal - enzymology; Muscles; Nucleotide sequence; Outer membrane protein; Outer membrane proteins; Phosphopyruvate hydratase; Phosphopyruvate Hydratase - immunology; Polyacrylamide; Polyclonal antibodies; Proteins; Pseudomonas aeruginosa; Pseudomonas aeruginosa - immunology; Rabbits; Skeletal muscle; Strains (organisms); Thromboangiitis Obliterans - immunology; Enolase; Affinity chromatography; Cross-reactivity; Outer membrane protein; Enterobacteriaceae; Human; Membrane protein; Microbiology; Enzyme; Antibody; Lyases; External membrane; Cell wall; Immunology; Cross reaction; Bacteria; Muscle; Carbon-oxygen lyases; Phosphopyruvate hydratase; Hydro-lyases

Enolase, is a glycolytic enzyme ubiquitous in higher organisms, where it forms tissue specific dimers of isoforms, also found in the cytoplasm of fermentative bacteria. The aim of this work was to identify enolase-like proteins in the cell wall of some Gram-negative bacteria using antibodies against...