PtWRKY14基因转化烟草及对TMV的抗性影响研究

PtWRKY14是从毛白杨中获得的WRKY基因,为研究PtWRKY14基因对植物抗病性的影响,构建了毛白杨PtWRKy 14基因的植物表达载体pBI121-W14,通过农杆菌介导的叶盘转化法转化烟草,PCR与Southern杂交进行转基因烟草鉴定.对获得的转基因烟草植株进行烟草花叶病毒（Tobacco mosaicvirus,TMV）的接种试验,结果表明PtWRKY14基因的转化使烟草对TMV的抗性增强.荧光定量PCR检测结果表明,TMV接种后,转基因烟草中PtWRKY14的表达量显著上调,同时转基因烟草中超氧化物歧化酶（Superoxide Dismutase,SOD）、过氧化物酶（Pero...

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Bibliographic Details
Published in广东农业科学 Vol. 41; no. 7; pp. 130 - 133
Main Author 王兴 林善枝
Format Journal Article
LanguageChinese
Published 北京林业大学生命科学与技术学院,北京,100083 2014
Subjects
PtWRKY14
抗病性
烟草花叶病毒
转基因烟草
烟草花叶病毒
转基因烟草
抗病性
PtWRKY14
Online AccessGet full text
ISSN1004-874X

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Summary:PtWRKY14是从毛白杨中获得的WRKY基因,为研究PtWRKY14基因对植物抗病性的影响,构建了毛白杨PtWRKy 14基因的植物表达载体pBI121-W14,通过农杆菌介导的叶盘转化法转化烟草,PCR与Southern杂交进行转基因烟草鉴定.对获得的转基因烟草植株进行烟草花叶病毒（Tobacco mosaicvirus,TMV）的接种试验,结果表明PtWRKY14基因的转化使烟草对TMV的抗性增强.荧光定量PCR检测结果表明,TMV接种后,转基因烟草中PtWRKY14的表达量显著上调,同时转基因烟草中超氧化物歧化酶（Superoxide Dismutase,SOD）、过氧化物酶（Peroxidase,POD）、过氧化氢酶（Catalase,CAT）的表达量均高于对照组.因此,PtWRKY14可能通过上调SOD、POD、CAT的表达从而增强植物抗病性.
Bibliography:44-1267/S
PtWRKY14; transgenic tobacco; tobacco mosaic virus; disease resistance
PtWRKY14 is a WRKY gene from Populus tomentosa. The study was conducted to investigate the effects of PtWRKY14 on plant disease resistance. The gene PtWRKY14 was constructed into binary vector pBI121 and transformed into tobacco plants by Agrobacterium-mediated leaf disc transformation. Transgenic tobaccos were identified by PCR and Southern hybridization. The results of tobacco mosaic virus （TMV） infection test showed that transgenic tobacco possessed certain resistance to TMV infection. The expression of PtWRKY14, superoxide Dismutase （SOD）, peroxidase （POD） and catalase （CAT） were analyzed by real-time PCR. The result showed PtWRKY14 was up-regulated expression by TMV treatment. While the expressions of SOD, POD and CAT in transgenic tobacco were more than in blank control tobacco. These results suggested that PtWRKY14 might be up-regulate expression of SOD, POD and CAT, leading to enhanced plant disease resistance.
WANG Xing, LI
ISSN:1004-874X