Loss of Claudins 2 and 15 From Mice Causes Defects in Paracellular Na+ Flow and Nutrient Transport in Gut and Leads to Death from Malnutrition

• Published in: Gastroenterology (New York, N.Y. 1943) Vol. 144; no. 2; pp. 369 - 380
• Main Authors: Wada, Masami, Tamura, Atsushi, Takahashi, Nobuyuki, Tsukita, Sachiko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2013
• Subjects: Amino Acids - pharmacokinetics; Animals; Biological Transport; Cell Membrane - metabolism; Cell Membrane - ultrastructure; Cell Membrane Permeability; Claudin-15; Claudin-2; Claudin-2 - genetics; Claudin-2 - metabolism; Claudins - genetics; Claudins - metabolism; Disease Models, Animal; Epithelial Cells - metabolism; Epithelial Cells - ultrastructure; Fats - pharmacokinetics; Gastroenterology and Hepatology; Glucose - pharmacokinetics; Immunoblotting; Intestinal Absorption - physiology; Intestinal Mucosa - metabolism; Intestinal Mucosa - ultrastructure; Intestine, Small - metabolism; Intestine, Small - ultrastructure; Malnutrition - genetics; Malnutrition - metabolism; Malnutrition - mortality; Mice; Mice, Knockout; Microscopy, Electron; Microscopy, Fluorescence; Na+-Dependent Transporter; Nutrient Malabsorption; Phenotype; Real-Time Polymerase Chain Reaction; Sodium - metabolism; Tight Junctions - genetics; Tight Junctions - metabolism; FFA; Claudin-15; pnd; qRT-PCR; Nutrient Malabsorption; DKO; mRNA; Claudin-2; Na+-Dependent Transporter; pAb; DHet; TG; Isc; OGTT; WT; triglyceride; oral glucose tolerance test; double-heterogenous; short-circuit current; Na +-Dependent Transporter; messenger RNA; polyclonal antibody; postnatal day; double-knockout; quantitative real-time reverse-transcription polymerase chain reaction; free fatty acid; wild type

The intestinal symport system moves nutrients across membranes via transporters, and is required for absorption of major nutrients such as glucose, amino acids, and bile acids (which are required for fat absorption). Most of these transporters are regulated by Na+, but the standard diet does not pro...