Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata

• Published in: Carbohydrate polymers Vol. 103; pp. 244 - 249
• Main Authors: Cheng, Bao-Hui, Chan, Judy Yuet-Wa, Chan, Ben Chung-Lap, Lin, Huang-Quan, Han, Xiao-Qiang, Zhou, Xuelin, Wan, David Chi-Cheong, Wang, Yi-Fen, Leung, Ping-Chung, Fung, Kwok-Pui, Lau, Clara Bik-San
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.03.2014; Elsevier
• Subjects: acid hydrolysis; adaptive immunity; agarose; Applied sciences; bioactive properties; Biological and medical sciences; Carbohydrate Conformation; chemokine CCL3; chemokine CCL4; chemokine CCL5; Cytokines - biosynthesis; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; esterification; Exact sciences and technology; Fourier transform infrared spectroscopy; HCP-2; high performance liquid chromatography; Houttuynia - chemistry; Houttuynia cordata; Humans; Immunomodulation; Immunomodulators; innate immunity; interleukin-1beta; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Medical sciences; Molecular Weight; Natural polymers; necrosis; nuclear magnetic resonance spectroscopy; Pectic polysaccharide; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Starch and polysaccharides; Structural characterization; Structure-Activity Relationship; T-lymphocytes; tumor necrosis factor-alpha; Houttuynia cordata; HCP-2; Immunomodulation; Pectic polysaccharide; Structural characterization; Molecular structure; Medicinal plant; Chinese medicine; Folk medicine; Dicotyledones; Angiospermae; Tumor necrosis factor α; Interleukin 1β; Macrophage inflammatory protein 1alpha; Experimental study; In vitro; Macrophage inflammatory protein 2; Biological activity; Pectic substance; Immunomodulator; Water soluble compound; Saururaceae; Plant origin; Oside polymer; Spermatophyta

•A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata.•HCP-2 was chemically characterized using acid hydrolysis, PMP derivation, FTIR and NMR.•HCP-2 exhibited immunomodulatory effects in vitro. Immunomodulation of natural polysaccharides has been the h...