Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata
•A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata.•HCP-2 was chemically characterized using acid hydrolysis, PMP derivation, FTIR and NMR.•HCP-2 exhibited immunomodulatory effects in vitro. Immunomodulation of natural polysaccharides has been the h...
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| Published in | Carbohydrate polymers Vol. 103; pp. 244 - 249 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Kidlington
Elsevier Ltd
15.03.2014
Elsevier |
| Subjects | |
| Online Access | Get full text |
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| Abstract | •A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata.•HCP-2 was chemically characterized using acid hydrolysis, PMP derivation, FTIR and NMR.•HCP-2 exhibited immunomodulatory effects in vitro.
Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-d-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. |
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| AbstractList | Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-500 [corrected] HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-d-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. • A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata . • HCP-2 was chemically characterized using acid hydrolysis, PMP derivation, FTIR and NMR. • HCP-2 exhibited immunomodulatory effects in vitro . Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α- d -galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-500 [corrected] HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer.Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-500 [corrected] HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked alpha -d-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1 beta (IL-1 beta ), tumor necrosis factor- alpha (TNF- alpha ), macrophage inhibitory protein-1 alpha (MIP-1 alpha ), macrophage inhibitory protein-1 beta (MIP-1 beta ), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. •A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata.•HCP-2 was chemically characterized using acid hydrolysis, PMP derivation, FTIR and NMR.•HCP-2 exhibited immunomodulatory effects in vitro. Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-d-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. |
| Author | Zhou, Xuelin Chan, Judy Yuet-Wa Chan, Ben Chung-Lap Fung, Kwok-Pui Wan, David Chi-Cheong Cheng, Bao-Hui Han, Xiao-Qiang Wang, Yi-Fen Lin, Huang-Quan Lau, Clara Bik-San Leung, Ping-Chung |
| Author_xml | – sequence: 1 givenname: Bao-Hui surname: Cheng fullname: Cheng, Bao-Hui organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 2 givenname: Judy Yuet-Wa surname: Chan fullname: Chan, Judy Yuet-Wa organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 3 givenname: Ben Chung-Lap surname: Chan fullname: Chan, Ben Chung-Lap organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 4 givenname: Huang-Quan surname: Lin fullname: Lin, Huang-Quan organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 5 givenname: Xiao-Qiang surname: Han fullname: Han, Xiao-Qiang organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 6 givenname: Xuelin surname: Zhou fullname: Zhou, Xuelin organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 7 givenname: David Chi-Cheong surname: Wan fullname: Wan, David Chi-Cheong organization: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 8 givenname: Yi-Fen surname: Wang fullname: Wang, Yi-Fen organization: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China – sequence: 9 givenname: Ping-Chung surname: Leung fullname: Leung, Ping-Chung organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 10 givenname: Kwok-Pui surname: Fung fullname: Fung, Kwok-Pui organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region – sequence: 11 givenname: Clara Bik-San surname: Lau fullname: Lau, Clara Bik-San email: claralau@cuhk.edu.hk organization: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Special Administrative Region |
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| Keywords | Houttuynia cordata HCP-2 Immunomodulation Pectic polysaccharide Structural characterization Molecular structure Medicinal plant Chinese medicine Folk medicine Dicotyledones Angiospermae Tumor necrosis factor α Interleukin 1β Macrophage inflammatory protein 1alpha Experimental study In vitro Macrophage inflammatory protein 2 Biological activity Pectic substance Immunomodulator Water soluble compound Saururaceae Plant origin Oside polymer Spermatophyta |
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| Snippet | •A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata.•HCP-2 was chemically characterized using acid... Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia... • A pure pectic polysaccharide (named HCP-2) was obtained from the water extract of Houttuynia cordata . • HCP-2 was chemically characterized using acid... |
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| SubjectTerms | acid hydrolysis adaptive immunity agarose Applied sciences bioactive properties Biological and medical sciences Carbohydrate Conformation chemokine CCL3 chemokine CCL4 chemokine CCL5 Cytokines - biosynthesis Dose-Response Relationship, Drug Drugs, Chinese Herbal - chemistry Drugs, Chinese Herbal - isolation & purification Drugs, Chinese Herbal - pharmacology esterification Exact sciences and technology Fourier transform infrared spectroscopy HCP-2 high performance liquid chromatography Houttuynia - chemistry Houttuynia cordata Humans Immunomodulation Immunomodulators innate immunity interleukin-1beta Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Medical sciences Molecular Weight Natural polymers necrosis nuclear magnetic resonance spectroscopy Pectic polysaccharide Pharmacology. Drug treatments Physicochemistry of polymers Polysaccharides - chemistry Polysaccharides - isolation & purification Polysaccharides - pharmacology Starch and polysaccharides Structural characterization Structure-Activity Relationship T-lymphocytes tumor necrosis factor-alpha |
| Title | Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata |
| URI | https://dx.doi.org/10.1016/j.carbpol.2013.12.048 https://www.ncbi.nlm.nih.gov/pubmed/24528726 https://www.proquest.com/docview/1500682588 https://www.proquest.com/docview/1520367548 https://www.proquest.com/docview/2000296462 https://pubmed.ncbi.nlm.nih.gov/PMC7112369 |
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