A long road/read to rapid high-resolution HLA typing: The nanopore perspective

• Published in: Human immunology Vol. 82; no. 7; pp. 488 - 495
• Main Author: Liu, Chang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2021
• Subjects: Alleles; Consensus sequence; Histocompatibility Testing - methods; HLA Antigens - genetics; Human leukocyte antigen; Humans; Immunogenetics - methods; Nanopore Sequencing; Next-generation sequencing; Sequencing error; PRG; Consensus sequence; Next-generation sequencing; ONT; Nanopore sequencing; INC-Seq; Human leukocyte antigen; SNV; SMRT; NGS; HLA; Sequencing error; PCR

Next-generation sequencing (NGS) has been widely adopted for clinical HLA typing and advanced immunogenetics researches. Current methodologies still face challenges in resolving cis–trans ambiguity involving distant variant positions, and the turnaround time is affected by testing volume and batchin...