The effect of peptide adsorption on signal linearity and a simple approach to improve reliability of quantification

• Published in: Journal of proteomics Vol. 85; pp. 160 - 164
• Main Authors: Warwood, Stacey, Byron, Adam, Humphries, Martin J., Knight, David
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24.06.2013; Elsevier
• Subjects: Abundance; adsorption; analysis; bioinformatics; chemistry; Hydrophobic and Hydrophilic Interactions; hydrophobicity; isotope labeling; Linearity; Mass Spectrometry; Mass Spectrometry - methods; methods; Models, Chemical; monitoring; Peptide; peptides; Peptides - analysis; Peptides - chemistry; proteins; proteomics; Quantification; signal peptide; stable isotopes; Technical Note; variance; Quantification; Linearity; MS; MRM; Abundance; v/v; Peptide

Peptide quantification using MS often relies on the comparison of peptide signal intensities between different samples, which is based on the assumption that observed signal intensity has a linear relationship to peptide abundance. A typical proteomics experiment is subject to multiple sources of va...