RNA is a Structural Element in Retrovirus Particles
A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle, this RNA species is unnecessary for particle assembly in vivo. In vitro, nucleic acids profoundly enhance the efficiency of assembly by recom...
Saved in:
| Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 98; no. 9; pp. 5246 - 5251 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
National Academy of Sciences
24.04.2001
National Acad Sciences The National Academy of Sciences |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle, this RNA species is unnecessary for particle assembly in vivo. In vitro, nucleic acids profoundly enhance the efficiency of assembly by recombinant Gag proteins, apparently by acting as "scaffolding" in the particle. To address the participation of RNA in retrovirus assembly in vivo, we analyzed murine leukemia virus particles that lack genomic RNA because of a deletion in the packaging signal of the viral RNA. We found that these particles contain cellular mRNA in place of genomic RNA. This result was particularly evident when Gag was expressed by using a Semliki Forest virus-derived vector: under these conditions, the Semliki Forest virus vector-directed mRNA became very abundant in the cells and was readily identified in the retroviral virus-like particles. Furthermore, we found that the retroviral cores were disrupted by treatment with RNase. Taken together, the data strongly suggest that RNA is a structural element in retrovirus particles. |
|---|---|
| AbstractList | A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle, this RNA species is unnecessary for particle assembly in vivo. In vitro, nucleic acids profoundly enhance the efficiency of assembly by recombinant Gag proteins, apparently by acting as "scaffolding" in the particle. To address the participation of RNA in retrovirus assembly in vivo, we analyzed murine leukemia virus particles that lack genomic RNA because of a deletion in the packaging signal of the viral RNA. We found that these particles contain cellular mRNA in place of genomic RNA. This result was particularly evident when Gag was expressed by using a Semliki Forest virus-derived vector: under these conditions, the Semliki Forest virus vector-directed mRNA became very abundant in the cells and was readily identified in the retroviral virus-like particles. Furthermore, we found that the retroviral cores were disrupted by treatment with RNase. Taken together, the data strongly suggest that RNA is a structural element in retrovirus particles. A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle, this RNA species is unnecessary for particle assembly in vivo . In vitro , nucleic acids profoundly enhance the efficiency of assembly by recombinant Gag proteins, apparently by acting as “scaffolding” in the particle. To address the participation of RNA in retrovirus assembly in vivo , we analyzed murine leukemia virus particles that lack genomic RNA because of a deletion in the packaging signal of the viral RNA. We found that these particles contain cellular mRNA in place of genomic RNA. This result was particularly evident when Gag was expressed by using a Semliki Forest virus-derived vector: under these conditions, the Semliki Forest virus vector-directed mRNA became very abundant in the cells and was readily identified in the retroviral virus-like particles. Furthermore, we found that the retroviral cores were disrupted by treatment with RNase. Taken together, the data strongly suggest that RNA is a structural element in retrovirus particles. A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle, this RNA species is unnecessary for particle assembly in vivo . In vitro , nucleic acids profoundly enhance the efficiency of assembly by recombinant Gag proteins, apparently by acting as “scaffolding” in the particle. To address the participation of RNA in retrovirus assembly in vivo , we analyzed murine leukemia virus particles that lack genomic RNA because of a deletion in the packaging signal of the viral RNA. We found that these particles contain cellular mRNA in place of genomic RNA. This result was particularly evident when Gag was expressed by using a Semliki Forest virus-derived vector: under these conditions, the Semliki Forest virus vector-directed mRNA became very abundant in the cells and was readily identified in the retroviral virus-like particles. Furthermore, we found that the retroviral cores were disrupted by treatment with RNase. Taken together, the data strongly suggest that RNA is a structural element in retrovirus particles. murine leukemia virus packaging cell lines retrovirus assembly RNA packaging Semliki Forest virus gene expression system A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle, this RNA species is unnecessary for particle assembly in vivo. |
| Author | Harvin, Demetria Mirro, Jane Rein, Alan Muriaux, Delphine |
| AuthorAffiliation | HIV Drug Resistance Program, National Cancer Institute–Frederick Cancer Research and Development Center, Frederick, MD 21702-1201 |
| AuthorAffiliation_xml | – name: HIV Drug Resistance Program, National Cancer Institute–Frederick Cancer Research and Development Center, Frederick, MD 21702-1201 |
| Author_xml | – sequence: 1 givenname: Delphine surname: Muriaux fullname: Muriaux, Delphine – sequence: 2 givenname: Jane surname: Mirro fullname: Mirro, Jane – sequence: 3 givenname: Demetria surname: Harvin fullname: Harvin, Demetria – sequence: 4 givenname: Alan surname: Rein fullname: Rein, Alan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/11320254$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkc1PGzEQxS1EBSH02hOgVQ-9bTr-WtsSF4RCWwm1FdCz5XW8ZaPNOtheRP97HCWkwAFOo9H7vdGz3wHa7X3vEPqEYYJB0K_L3sQJKAwAVMkdNMJ5KSumYBeNAIgoJSNsHx3EOM-M4hL20D7GlADhbITo1c-zoo2FKa5TGGwagumKaecWrk9F2xdXLgV_34YhFr9NSK3tXDxEHxrTRfdxM8foz8X05vx7efnr24_zs8vScklSyRQTzjFbCeKsqLlQFGphZlJxVlFuBMVGMcMJiBxmJgzhNYdGNbYmKjvoGJ2u7y6HeuFmNkfK6fQytAsT_mlvWv1S6dtb_dffa0qx4tn-ZWMP_m5wMelFG63rOtM7P0QtQGIhsXwXzBTL91gGP78C534Iff4DTQBTRSRdpZ6sIRt8jME128AY9KozvepMbzvLhpPnz_yPb0p6lm9lfJKV1EpzwirdDF2X3EPK4PFbYNaP1vo8Jh-2AAXOuaroI6-6s4s |
| CitedBy_id | crossref_primary_10_3390_v2040939 crossref_primary_10_1088_1367_2630_15_3_035028 crossref_primary_10_1038_nrmicro1210 crossref_primary_10_1371_journal_pone_0083874 crossref_primary_10_1128_JVI_76_22_11757_11762_2002 crossref_primary_10_1073_pnas_0305665101 crossref_primary_10_1128_JVI_01473_12 crossref_primary_10_1128_JVI_02031_08 crossref_primary_10_1097_00002030_200216004_00003 crossref_primary_10_1089_aid_2010_0137 crossref_primary_10_1111_j_1348_0421_2009_00164_x crossref_primary_10_1007_s13238_017_0461_z crossref_primary_10_1517_14712598_2013_779249 crossref_primary_10_1128_JVI_00106_18 crossref_primary_10_1097_00002030_200100005_00003 crossref_primary_10_1006_viro_2002_1578 crossref_primary_10_1128_JVI_01880_07 crossref_primary_10_1128_JVI_79_14_8732_8741_2005 crossref_primary_10_1073_pnas_2112475118 crossref_primary_10_1006_jmbi_2001_5139 crossref_primary_10_1128_JVI_02833_06 crossref_primary_10_1128_JVI_00743_11 crossref_primary_10_1073_pnas_0907364106 crossref_primary_10_3390_v12101115 crossref_primary_10_1080_13102818_2005_10817147 crossref_primary_10_1128_JVI_76_9_4331_4340_2002 crossref_primary_10_1128_JVI_00749_09 crossref_primary_10_1016_j_vetimm_2009_10_003 crossref_primary_10_1128_mBio_00341_10 crossref_primary_10_1186_1472_6750_13_35 crossref_primary_10_1016_j_virol_2012_05_003 crossref_primary_10_1128_JVI_76_23_11853_11865_2002 crossref_primary_10_1016_j_virusres_2012_06_001 crossref_primary_10_1016_S0022_2836_02_01305_0 crossref_primary_10_1186_1743_422X_10_129 crossref_primary_10_3390_v8080235 crossref_primary_10_1128_JVI_77_11_6284_6292_2003 crossref_primary_10_4161_rna_8_4_16030 crossref_primary_10_1093_nar_gkm153 crossref_primary_10_1128_JVI_02545_08 crossref_primary_10_3389_fgene_2015_00108 crossref_primary_10_1186_1742_4690_7_29 crossref_primary_10_1128_JVI_00124_12 crossref_primary_10_1016_j_jmb_2018_04_042 crossref_primary_10_1073_pnas_1408037111 crossref_primary_10_1021_jm201442t crossref_primary_10_1155_2012_979765 crossref_primary_10_1016_j_virol_2007_09_017 crossref_primary_10_1128_JVI_77_17_9431_9438_2003 crossref_primary_10_1083_jcb_201504006 crossref_primary_10_1128_JVI_79_3_1803_1812_2005 crossref_primary_10_1016_j_febslet_2009_11_038 crossref_primary_10_3390_v13101946 crossref_primary_10_1016_j_isci_2024_109107 crossref_primary_10_1016_S1097_2765_02_00474_4 crossref_primary_10_1128_JVI_00355_06 crossref_primary_10_1128_JVI_78_20_10927_10938_2004 crossref_primary_10_1128_JVI_00862_11 crossref_primary_10_1042_BC20080025 crossref_primary_10_1093_nar_gkab1282 crossref_primary_10_1128_JVI_00189_08 crossref_primary_10_1089_088922202320567851 crossref_primary_10_1016_j_jmb_2012_01_033 crossref_primary_10_1016_j_virol_2008_07_015 crossref_primary_10_1016_j_jviromet_2015_07_023 crossref_primary_10_1128_JVI_79_23_14498_14506_2005 crossref_primary_10_1016_j_bbrc_2009_03_150 crossref_primary_10_1128_JVI_77_1_754_761_2003 crossref_primary_10_1128_JVI_00040_13 crossref_primary_10_3390_v14030478 crossref_primary_10_1128_JVI_00664_06 crossref_primary_10_1099_vir_0_065151_0 crossref_primary_10_1128_JVI_00750_15 crossref_primary_10_1016_j_jmb_2008_12_073 crossref_primary_10_1021_bi101245j crossref_primary_10_1038_s41598_018_34536_y crossref_primary_10_1186_1742_4690_3_96 crossref_primary_10_1016_j_omtn_2021_12_033 crossref_primary_10_1261_rna_057299_116 crossref_primary_10_1128_JVI_00821_17 crossref_primary_10_1128_JVI_06979_11 crossref_primary_10_3390_v15030690 crossref_primary_10_1016_j_jmb_2015_01_015 crossref_primary_10_1128_mBio_02025_15 crossref_primary_10_1186_s12977_018_0417_2 crossref_primary_10_1128_JVI_02303_08 crossref_primary_10_1016_j_jmb_2005_09_071 crossref_primary_10_1261_rna_2306306 crossref_primary_10_4161_rna_7_6_13777 crossref_primary_10_1186_1742_4690_4_90 crossref_primary_10_4161_rna_7_6_14065 crossref_primary_10_1002_bip_20622 crossref_primary_10_1073_pnas_062652199 crossref_primary_10_1261_rna_038869_113 crossref_primary_10_1016_j_virusres_2007_12_006 crossref_primary_10_1111_febs_15618 crossref_primary_10_1021_cb500316h crossref_primary_10_1038_gt_2008_159 crossref_primary_10_1016_j_virusres_2012_09_008 crossref_primary_10_1128_JVI_77_14_7890_7902_2003 crossref_primary_10_1038_nrmicro903 crossref_primary_10_1186_s12977_016_0287_4 crossref_primary_10_1016_j_tibs_2011_04_001 crossref_primary_10_3390_v3060770 crossref_primary_10_1021_acs_jproteome_0c00581 crossref_primary_10_1128_JVI_78_19_10390_10398_2004 crossref_primary_10_1016_j_jmb_2011_04_029 crossref_primary_10_3390_v8070192 crossref_primary_10_1016_j_bpj_2023_11_017 crossref_primary_10_1021_jacs_2c02568 crossref_primary_10_1073_pnas_1006897107 crossref_primary_10_1128_JVI_00006_11 crossref_primary_10_1128_JVI_76_16_8485_8493_2002 crossref_primary_10_1128_JVI_02319_16 crossref_primary_10_1128_JVI_03513_13 crossref_primary_10_1128_JVI_00035_10 crossref_primary_10_1128_JVI_02022_09 crossref_primary_10_1128_MMBR_00040_08 crossref_primary_10_1074_jbc_M507255200 crossref_primary_10_1128_JVI_00099_09 crossref_primary_10_1128_JVI_76_22_11177_11185_2002 crossref_primary_10_1186_s12977_014_0087_7 crossref_primary_10_1128_JVI_79_22_14131_14140_2005 crossref_primary_10_1093_nar_gkab223 crossref_primary_10_1128_JVI_77_5_2922_2927_2003 crossref_primary_10_1128_JVI_79_21_13528_13537_2005 crossref_primary_10_1016_j_jmb_2021_167293 crossref_primary_10_1016_j_chom_2016_02_004 crossref_primary_10_7554_eLife_27055 crossref_primary_10_1128_JVI_78_21_12058_12061_2004 crossref_primary_10_2222_jsv_61_91 crossref_primary_10_1097_01_COH_0000221591_11294_c1 crossref_primary_10_1128_JVI_77_17_9388_9398_2003 crossref_primary_10_7554_eLife_84881 crossref_primary_10_1002_rmv_570 crossref_primary_10_3390_v8090243 crossref_primary_10_1073_pnas_1805728115 crossref_primary_10_1128_JVI_00288_11 crossref_primary_10_1128_JVI_78_22_12378_12385_2004 crossref_primary_10_1073_pnas_0602818103 crossref_primary_10_1016_j_virol_2005_08_031 crossref_primary_10_1227_NEU_0b013e3182846e63 crossref_primary_10_1074_jbc_M109_019844 crossref_primary_10_1021_bp049865x crossref_primary_10_3390_v8090248 crossref_primary_10_1128_JVI_78_18_9675_9688_2004 crossref_primary_10_3390_v8090246 crossref_primary_10_1186_1742_4690_4_48 crossref_primary_10_1177_135965350701200807 crossref_primary_10_1051_medsci_20153105014 crossref_primary_10_1111_apha_13886 crossref_primary_10_1016_j_virol_2004_06_019 crossref_primary_10_1186_1742_4690_4_54 crossref_primary_10_1371_journal_ppat_1006221 crossref_primary_10_1016_j_jmb_2006_04_017 crossref_primary_10_1016_j_virusres_2012_08_014 crossref_primary_10_1111_j_1365_2443_2010_01476_x crossref_primary_10_1128_JVI_78_16_8486_8495_2004 crossref_primary_10_1128_JVI_77_15_8329_8335_2003 crossref_primary_10_1016_j_jmb_2011_04_072 crossref_primary_10_1134_S0026893312020148 crossref_primary_10_1186_1742_4690_4_37 crossref_primary_10_1016_j_cell_2014_09_057 crossref_primary_10_1128_JVI_79_22_13839_13847_2005 crossref_primary_10_1128_JVI_01016_08 crossref_primary_10_1016_j_virol_2008_02_026 crossref_primary_10_1371_journal_ppat_1001200 crossref_primary_10_1016_j_jmb_2009_11_033 crossref_primary_10_1128_JVI_02831_06 crossref_primary_10_1128_JVI_78_3_1411_1420_2004 crossref_primary_10_1016_j_jmb_2003_10_059 crossref_primary_10_1128_JVI_02049_07 crossref_primary_10_1128_JVI_76_8_4131_4137_2002 crossref_primary_10_1128_JVI_00220_18 crossref_primary_10_1128_JVI_01912_07 crossref_primary_10_1016_j_virusres_2014_05_011 crossref_primary_10_1186_1742_4690_3_18 crossref_primary_10_1038_mt_2016_143 crossref_primary_10_1111_j_1600_0854_2006_00461_x crossref_primary_10_1128_JVI_76_11_5452_5462_2002 crossref_primary_10_1128_JVI_76_22_11405_11413_2002 crossref_primary_10_1128_JVI_00699_09 crossref_primary_10_1016_j_virol_2003_09_008 crossref_primary_10_1099_jgv_0_000736 crossref_primary_10_1097_01_COH_0000221592_49412_1f crossref_primary_10_1128_JVI_00627_06 crossref_primary_10_3390_ijms22169103 crossref_primary_10_1128_JVI_76_7_3089_3094_2002 crossref_primary_10_3390_v13091788 crossref_primary_10_1128_JVI_77_3_2010_2020_2003 crossref_primary_10_1016_j_jmb_2006_08_070 crossref_primary_10_1128_JVI_02694_05 crossref_primary_10_1128_JVI_78_17_9423_9430_2004 crossref_primary_10_3934_biophy_2015_3_343 crossref_primary_10_1002_smll_201601712 crossref_primary_10_3390_v10050261 crossref_primary_10_4161_rna_7_6_14117 crossref_primary_10_4049_jimmunol_178_9_5839 crossref_primary_10_4161_rna_7_6_14115 crossref_primary_10_1006_viro_2002_1532 crossref_primary_10_1128_JVI_01219_09 crossref_primary_10_1073_pnas_0906822106 crossref_primary_10_1016_j_jinorgbio_2022_112117 crossref_primary_10_1016_j_jmb_2006_08_065 crossref_primary_10_1080_15476286_2018_1481696 crossref_primary_10_1016_j_semcdb_2018_03_015 crossref_primary_10_1261_rna_678307 crossref_primary_10_1128_JVI_05889_11 crossref_primary_10_1016_j_jmb_2006_10_072 crossref_primary_10_1039_C8RA02084A crossref_primary_10_1016_j_jmb_2013_11_025 crossref_primary_10_1016_j_virusres_2014_06_009 crossref_primary_10_3390_ijms222212398 crossref_primary_10_1073_pnas_191224698 crossref_primary_10_3389_fmicb_2014_00253 crossref_primary_10_1074_jbc_RA119_010580 crossref_primary_10_1128_JVI_05191_11 crossref_primary_10_1051_medsci_200824149 crossref_primary_10_1186_1743_422X_5_162 crossref_primary_10_1128_JVI_00714_10 crossref_primary_10_1128_JVI_78_2_716_723_2004 crossref_primary_10_1128_JVI_01357_07 crossref_primary_10_1128_JVI_77_10_5547_5556_2003 crossref_primary_10_1016_j_jmb_2006_09_081 crossref_primary_10_3390_v8100276 crossref_primary_10_1093_nar_gkq1314 crossref_primary_10_1126_sciadv_adk8297 crossref_primary_10_1016_j_virol_2004_03_023 crossref_primary_10_1186_1743_422X_8_462 crossref_primary_10_1128_JVI_00544_15 crossref_primary_10_1186_1742_4690_8_6 crossref_primary_10_1016_S0042_6822_03_00586_5 crossref_primary_10_1016_j_virol_2008_05_010 |
| Cites_doi | 10.1016/0092-8674(78)90245-3 10.1128/jvi.39.2.471-480.1981 10.1128/JVI.72.11.9034-9044.1998 10.1128/mcb.6.8.2895-2902.1986 10.1016/0022-2836(86)90451-1 10.1128/jvi.71.9.6940-6946.1997 10.1128/jvi.36.3.692-700.1980 10.1128/JVI.73.8.7050-7055.1999 10.1128/jvi.71.9.6765-6776.1997 10.1128/jvi.68.9.6124-6129.1994 10.1016/0092-8674(86)90071-1 10.1128/JVI.73.5.4485-4488.1999 10.1128/jvi.70.10.6607-6616.1996 10.1073/pnas.74.8.3446 10.1128/jvi.65.1.71-80.1991 10.1128/JVI.72.3.1782-1789.1998 10.1128/jvi.67.4.1936-1944.1993 10.1128/JVI.72.3.1902-1909.1998 10.1073/pnas.95.13.7299 10.1016/0092-8674(89)90873-8 10.1128/JVI.73.3.2270-2279.1999 10.1073/pnas.93.21.11658 10.1016/S0065-3527(08)60022-6 10.1128/JVI.74.16.7431-7441.2000 10.1016/0042-6822(82)90359-2 10.1128/JVI.74.7.3046-3057.2000 10.1016/S0960-9822(06)00331-9 10.1021/bi991611a 10.1126/science.283.5398.80 10.1128/jvi.67.9.5443-5449.1993 10.1002/(SICI)1099-1654(199707)7:2<107::AID-RMV191>3.0.CO;2-M 10.1128/jvi.14.1.152-161.1974 10.1016/0092-8674(83)90344-6 10.1128/jvi.29.1.328-335.1979 10.1111/j.1432-1033.1997.t01-1-00592.x 10.1128/jvi.69.11.7319-7323.1995 10.1016/0042-6822(78)90332-X 10.1083/jcb.135.6.1841 10.1038/5799 10.1128/JVI.73.7.5309-5319.1999 10.1073/pnas.71.4.1154 10.1128/jvi.69.10.6487-6497.1995 10.1128/JVI.73.5.4251-4256.1999 |
| ContentType | Journal Article |
| Copyright | Copyright 1993-2001 National Academy of Sciences of the United States of America Copyright National Academy of Sciences Apr 24, 2001 Copyright © 2001, The National Academy of Sciences 2001 |
| Copyright_xml | – notice: Copyright 1993-2001 National Academy of Sciences of the United States of America – notice: Copyright National Academy of Sciences Apr 24, 2001 – notice: Copyright © 2001, The National Academy of Sciences 2001 |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7X8 5PM |
| DOI | 10.1073/pnas.091000398 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Virology and AIDS Abstracts Oncogenes and Growth Factors Abstracts Technology Research Database Nucleic Acids Abstracts Ecology Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Entomology Abstracts Genetics Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Immunology Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
| DatabaseTitleList | AIDS and Cancer Research Abstracts MEDLINE - Academic CrossRef MEDLINE Virology and AIDS Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) |
| EISSN | 1091-6490 |
| EndPage | 5251 |
| ExternalDocumentID | 73250281 10_1073_pnas_091000398 11320254 98_9_5246 3055596 |
| Genre | Journal Article Feature |
| GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 123 29P 2AX 2FS 2WC 3O- 4.4 53G 5RE 5VS 79B 85S AACGO AAFWJ AANCE AAYJJ ABBHK ABOCM ABPLY ABPPZ ABTLG ABXSQ ABZEH ACGOD ACIWK ACNCT ACPRK ADULT ADZLD AENEX AEUPB AEXZC AFDAS AFFNX AFOSN AFRAH ALMA_UNASSIGNED_HOLDINGS AQVQM ASUFR AS~ BKOMP CS3 D0L DCCCD DIK DNJUQ DOOOF DU5 DWIUU E3Z EBS EJD F20 F5P FRP GX1 HGD HH5 HQ3 HTVGU HYE JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST KQ8 L7B LU7 MVM N9A NEJ N~3 O9- OK1 P-O PNE PQQKQ R.V RHF RHI RNA RNS RPM RXW SA0 SJN TAE TN5 UKR VOH VQA W8F WH7 WHG WOQ WOW X7M XFK XSW Y6R YBH YKV YSK ZA5 ZCA ZCG ~02 ~KM - 02 08R 0R 1AW 55 AAPBV ABFLS ABPTK ADACO AJYGW AS DZ GJ KM OHM PQEST X XHC ADACV CGR CUY CVF ECM EIF H13 IPSME NPM AAYXX CITATION 7QG 7QL 7QP 7QR 7SN 7SS 7T5 7TK 7TM 7TO 7U9 8FD C1K FR3 H94 M7N P64 RC3 7X8 5PM |
| ID | FETCH-LOGICAL-c582t-4947ee4c672ec7b57930b7ad8954635a731a94a5207202d7a25b50f9fcb29ec73 |
| IEDL.DBID | RPM |
| ISSN | 0027-8424 |
| IngestDate | Tue Sep 17 21:17:08 EDT 2024 Sat Aug 17 03:41:56 EDT 2024 Sat Aug 17 03:33:47 EDT 2024 Thu Oct 10 17:26:21 EDT 2024 Fri Aug 23 02:52:46 EDT 2024 Sat Sep 28 07:36:38 EDT 2024 Thu May 30 08:50:32 EDT 2019 Wed Nov 11 00:29:19 EST 2020 Fri Feb 02 07:05:24 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c582t-4947ee4c672ec7b57930b7ad8954635a731a94a5207202d7a25b50f9fcb29ec73 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 To whom reprint requests should be addressed at: HIV Drug Resistance Program, National Cancer Institute–Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201. E-mail: rein@ncifcrf.gov. Edited by John M. Coffin, Tufts University School of Medicine, Boston, MA, and approved February 23, 2001 |
| OpenAccessLink | https://doi.org/10.1073/pnas.091000398 |
| PMID | 11320254 |
| PQID | 201392837 |
| PQPubID | 42026 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_17849534 jstor_primary_3055596 pnas_primary_98_9_5246_fulltext pubmed_primary_11320254 proquest_journals_201392837 pnas_primary_98_9_5246 proquest_miscellaneous_70817818 pubmedcentral_primary_oai_pubmedcentral_nih_gov_33195 crossref_primary_10_1073_pnas_091000398 |
| ProviderPackageCode | RNA PNE |
| PublicationCentury | 2000 |
| PublicationDate | 20010424 2001-04-24 2001-Apr-24 |
| PublicationDateYYYYMMDD | 2001-04-24 |
| PublicationDate_xml | – month: 4 year: 2001 text: 20010424 day: 24 |
| PublicationDecade | 2000 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Washington |
| PublicationTitle | Proceedings of the National Academy of Sciences - PNAS |
| PublicationTitleAlternate | Proc Natl Acad Sci U S A |
| PublicationYear | 2001 |
| Publisher | National Academy of Sciences National Acad Sciences The National Academy of Sciences |
| Publisher_xml | – name: National Academy of Sciences – name: National Acad Sciences – name: The National Academy of Sciences |
| References | 9636143 - Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7299-304 9261401 - J Virol. 1997 Sep;71(9):6765-76 9368755 - Curr Biol. 1997 Oct 1;7(10):729-38 80281 - Cell. 1978 Jul;14(3):601-9 9872746 - Science. 1999 Jan 1;283(5398):80-3 1985218 - J Virol. 1991 Jan;65(1):71-80 7090184 - Virology. 1982 Apr 30;118(2):401-10 10196352 - J Virol. 1999 May;73(5):4485-8 9765448 - J Virol. 1998 Nov;72(11):9034-44 3785217 - Mol Cell Biol. 1986 Aug;6(8):2895-902 4134468 - J Virol. 1974 Jul;14(1):152-61 2676191 - Cell. 1989 Oct 6;59(1):103-12 10048920 - Nat Struct Biol. 1999 Feb;6(2):114-6 8876192 - Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11658-63 10545189 - Biochemistry. 1999 Nov 2;38(44):14644-52 10398476 - Rev Med Virol. 1997 Jul;7(2):107-122 716227 - Virology. 1978 Oct 1;90(1):54-9 8794295 - J Virol. 1996 Oct;70(10):6607-16 6268836 - J Virol. 1981 Aug;39(2):471-80 7666550 - J Virol. 1995 Oct;69(10):6487-97 9370371 - Eur J Biochem. 1997 Oct 15;249(2):592-600 10906196 - J Virol. 2000 Aug;74(16):7431-41 4924992 - Adv Virus Res. 1970;16:99-134 9261422 - J Virol. 1997 Sep;71(9):6940-6 219227 - J Virol. 1979 Jan;29(1):328-35 9499042 - J Virol. 1998 Mar;72(3):1902-9 4524626 - Proc Natl Acad Sci U S A. 1974 Apr;71(4):1154-8 7474162 - J Virol. 1995 Nov;69(11):7319-23 9971810 - J Virol. 1999 Mar;73(3):2270-9 3806677 - J Mol Biol. 1986 Oct 20;191(4):639-58 8791728 - Curr Top Microbiol Immunol. 1996;214:177-218 6162035 - J Virol. 1980 Dec;36(3):692-700 9499028 - J Virol. 1998 Mar;72(3):1782-9 410020 - Proc Natl Acad Sci U S A. 1977 Aug;74(8):3446-50 10364277 - J Virol. 1999 Jul;73(7):5309-19 6678608 - Cell. 1983 May;33(1):153-9 6286831 - J Mol Appl Genet. 1982;1(4):327-41 8383219 - J Virol. 1993 Apr;67(4):1936-44 8991095 - J Cell Biol. 1996 Dec;135(6 Pt 2):1841-52 8057495 - J Virol. 1994 Sep;68(9):6124-9 10708419 - J Virol. 2000 Apr;74(7):3046-57 3019556 - Cell. 1986 Sep 12;46(6):895-904 8350405 - J Virol. 1993 Sep;67(9):5443-9 10400808 - J Virol. 1999 Aug;73(8):7050-5 10196321 - J Virol. 1999 May;73(5):4251-6 e_1_3_3_18_2 e_1_3_3_39_2 e_1_3_3_37_2 e_1_3_3_14_2 e_1_3_3_10_2 e_1_3_3_31_2 e_1_3_3_40_2 Swanstrom R (e_1_3_3_12_2) 1997 e_1_3_3_5_2 e_1_3_3_7_2 e_1_3_3_9_2 Southern P J (e_1_3_3_27_2) 1982; 1 e_1_3_3_29_2 e_1_3_3_23_2 e_1_3_3_48_2 e_1_3_3_25_2 e_1_3_3_46_2 e_1_3_3_1_2 e_1_3_3_44_2 e_1_3_3_3_2 e_1_3_3_21_2 e_1_3_3_42_2 e_1_3_3_17_2 e_1_3_3_19_2 e_1_3_3_38_2 e_1_3_3_13_2 Schlesinger S (e_1_3_3_35_2) 1996 e_1_3_3_36_2 e_1_3_3_15_2 e_1_3_3_34_2 e_1_3_3_11_2 e_1_3_3_30_2 Berkowitz R (e_1_3_3_16_2) 1996; 214 Ekstrom M (e_1_3_3_32_2) 1998 Sambrook J (e_1_3_3_33_2) 1989 e_1_3_3_6_2 e_1_3_3_8_2 e_1_3_3_28_2 e_1_3_3_49_2 e_1_3_3_24_2 e_1_3_3_47_2 e_1_3_3_26_2 e_1_3_3_45_2 e_1_3_3_2_2 e_1_3_3_20_2 e_1_3_3_43_2 e_1_3_3_4_2 e_1_3_3_22_2 e_1_3_3_41_2 |
| References_xml | – ident: e_1_3_3_10_2 doi: 10.1016/0092-8674(78)90245-3 – ident: e_1_3_3_39_2 doi: 10.1128/jvi.39.2.471-480.1981 – ident: e_1_3_3_21_2 doi: 10.1128/JVI.72.11.9034-9044.1998 – ident: e_1_3_3_29_2 doi: 10.1128/mcb.6.8.2895-2902.1986 – ident: e_1_3_3_8_2 doi: 10.1016/0022-2836(86)90451-1 – ident: e_1_3_3_31_2 doi: 10.1128/jvi.71.9.6940-6946.1997 – ident: e_1_3_3_43_2 doi: 10.1128/jvi.36.3.692-700.1980 – ident: e_1_3_3_15_2 doi: 10.1128/JVI.73.8.7050-7055.1999 – ident: e_1_3_3_19_2 doi: 10.1128/jvi.71.9.6765-6776.1997 – ident: e_1_3_3_34_2 doi: 10.1128/jvi.68.9.6124-6129.1994 – ident: e_1_3_3_1_2 doi: 10.1016/0092-8674(86)90071-1 – ident: e_1_3_3_48_2 doi: 10.1128/JVI.73.5.4485-4488.1999 – ident: e_1_3_3_45_2 doi: 10.1128/jvi.70.10.6607-6616.1996 – ident: e_1_3_3_37_2 doi: 10.1073/pnas.74.8.3446 – ident: e_1_3_3_40_2 doi: 10.1128/jvi.65.1.71-80.1991 – ident: e_1_3_3_20_2 doi: 10.1128/JVI.72.3.1782-1789.1998 – ident: e_1_3_3_2_2 doi: 10.1128/jvi.67.4.1936-1944.1993 – ident: e_1_3_3_44_2 doi: 10.1128/JVI.72.3.1902-1909.1998 – ident: e_1_3_3_14_2 doi: 10.1073/pnas.95.13.7299 – volume: 1 start-page: 327 year: 1982 ident: e_1_3_3_27_2 publication-title: J Mol Appl Genet contributor: fullname: Southern P J – ident: e_1_3_3_11_2 doi: 10.1016/0092-8674(89)90873-8 – ident: e_1_3_3_24_2 doi: 10.1128/JVI.73.3.2270-2279.1999 – ident: e_1_3_3_30_2 doi: 10.1073/pnas.93.21.11658 – ident: e_1_3_3_5_2 doi: 10.1016/S0065-3527(08)60022-6 – ident: e_1_3_3_26_2 doi: 10.1128/JVI.74.16.7431-7441.2000 – ident: e_1_3_3_7_2 doi: 10.1016/0042-6822(82)90359-2 – ident: e_1_3_3_46_2 doi: 10.1128/JVI.74.7.3046-3057.2000 – ident: e_1_3_3_13_2 doi: 10.1016/S0960-9822(06)00331-9 – ident: e_1_3_3_4_2 doi: 10.1021/bi991611a – ident: e_1_3_3_25_2 doi: 10.1126/science.283.5398.80 – ident: e_1_3_3_28_2 doi: 10.1128/jvi.67.9.5443-5449.1993 – ident: e_1_3_3_3_2 doi: 10.1002/(SICI)1099-1654(199707)7:2<107::AID-RMV191>3.0.CO;2-M – ident: e_1_3_3_17_2 doi: 10.1128/jvi.14.1.152-161.1974 – start-page: 825 volume-title: Virology year: 1996 ident: e_1_3_3_35_2 contributor: fullname: Schlesinger S – volume-title: Molecular Cloning: A Laboratory Manual year: 1989 ident: e_1_3_3_33_2 contributor: fullname: Sambrook J – ident: e_1_3_3_18_2 doi: 10.1016/0092-8674(83)90344-6 – ident: e_1_3_3_42_2 doi: 10.1128/jvi.29.1.328-335.1979 – volume: 214 start-page: 177 year: 1996 ident: e_1_3_3_16_2 publication-title: Curr Top Microbiol Immunol contributor: fullname: Berkowitz R – ident: e_1_3_3_23_2 doi: 10.1111/j.1432-1033.1997.t01-1-00592.x – ident: e_1_3_3_41_2 doi: 10.1128/jvi.69.11.7319-7323.1995 – ident: e_1_3_3_6_2 doi: 10.1016/0042-6822(78)90332-X – ident: e_1_3_3_36_2 doi: 10.1083/jcb.135.6.1841 – ident: e_1_3_3_47_2 doi: 10.1038/5799 – start-page: 263 volume-title: Retroviruses year: 1997 ident: e_1_3_3_12_2 contributor: fullname: Swanstrom R – ident: e_1_3_3_9_2 doi: 10.1128/JVI.73.7.5309-5319.1999 – ident: e_1_3_3_38_2 doi: 10.1073/pnas.71.4.1154 – ident: e_1_3_3_22_2 doi: 10.1128/jvi.69.10.6487-6497.1995 – ident: e_1_3_3_49_2 doi: 10.1128/JVI.73.5.4251-4256.1999 – start-page: 218 volume-title: Cell Biology: A Laboratory Handbook year: 1998 ident: e_1_3_3_32_2 contributor: fullname: Ekstrom M |
| SSID | ssj0009580 |
| Score | 2.2299454 |
| Snippet | A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle,... A single retroviral protein, Gag, is sufficient for virus particle assembly. While Gag is capable of specifically packaging the genomic RNA into the particle,... |
| SourceID | pubmedcentral proquest crossref pubmed pnas jstor |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 5246 |
| SubjectTerms | Animals Biological Sciences Cell Line Cricetinae Gag protein Gene Products, gag - genetics Gene Products, gag - metabolism Genetic Vectors - genetics Genome, Viral Genomics Humans Leukemia Virus, Murine - chemistry Leukemia Virus, Murine - genetics Leukemia Virus, Murine - metabolism Messenger RNA Microbiology Molecules Murine leukemia virus Packaging Particle interactions Retroviridae Retrovirus Reverse Transcriptase Polymerase Chain Reaction ribonuclease Ribonuclease, Pancreatic - metabolism Ribonucleic acid RNA RNA, Messenger - analysis RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Viral - analysis RNA, Viral - genetics RNA, Viral - metabolism Semliki Forest virus Semliki forest virus - genetics Sequence Deletion - genetics Viral RNA Virion - chemistry Virion - genetics Virion - metabolism Virions Virus Assembly Viruses |
| Title | RNA is a Structural Element in Retrovirus Particles |
| URI | https://www.jstor.org/stable/3055596 http://www.pnas.org/content/98/9/5246.abstract https://www.ncbi.nlm.nih.gov/pubmed/11320254 https://www.proquest.com/docview/201392837 https://search.proquest.com/docview/17849534 https://search.proquest.com/docview/70817818 https://pubmed.ncbi.nlm.nih.gov/PMC33195 |
| Volume | 98 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELZoT1wQfUEoFB-QgEP2YY9j-1hVVC2oFUJU6s2yHUes1LqrZvv_GTvOlkXlwi2Kx1E8nrEn8TffEPJBBQ3gWFenasY1aLxyXHa1920r1Nw6NUv5zheXzdkVfL0W1yWPuy-wyujdYhJvbidx8StjK5e3fjrixKbfL0442o2YbpEtyfn4gb7m2VVD1gnDxRcYjDyNkk-X0faTtD2mfNRcpy-lDzMBG1vSgEpMVKco_lTY-Td68o_t6PQleVHiSHo8vO8OeRbiLtkpntrTT4VO-vMe4T8uj-mip5YOXLGJZ4OGATVOF5Heh1X6rXD_0NPlOPx9cnX65efJWV1KJdReKLZCJYMMAXwjWfDSCfS6mZO2VTrR3Qsr-dxqsILNJA65lZYJJ2ad7rxjGnvwA7Id72J4TagFEIKlOn-qAStbi2spt8I3ChyopqnIx1FbZjkwYph8ki25STozaxVXZD8rcy2W2cU0PuEgC463tTLaCAbY8P7pBtMVJExFDscJMcXZesNSGJtYfFL_sRW9JB192BjuHnozlyoBaeHfEhJjI4nRS0VeDdP7OLhiJhURGxO_FkgM3ZstaLiZqTsb6pv_7HdIng-AN6gZvCXbaCPhHUZAK3eEsf_5t6Ns-b8Bg5EEOg |
| link.rule.ids | 230,315,733,786,790,891,27955,27956,53825,53827 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB1BOcClpdDStEB9QAIOye4649g5VhXVAt0VQlupN8t2HLGCpqtm99JfX38kC1uVA9yizDiRMzP2JHnzBuCdsCWipnXquxmnWLojnfM6NaaqmBgpLYa-3nkyLcYX-OWSXXZ13G0Hq2yMnmfNr6usmf8I2MrFlRn0OLHBt8lp7vyGDR7DExetlPWv6GumXRHrTqhbfpFiz9TI88GiUW3mN0hfkRo69fkCYspwY1OKuERPdurUH0o87-Mn_9iQznZg1k8l4lB-ZqulzsztPZbHf5zrc9juElRyEoW78Mg2L2C3WwJa8qHjqf74EvLv0xMyb4kikYTWE3gQG-HoZN6QG7v03ytuVi1Z9Pfag4uzT7PTcdr1YEgNE3TprIfcWjQFp9ZwzVw4DzVXlSg9jz5TPB-pEhWjQ-6eZMUVZZoN67I2mpZuRL4PW811Yw-AKETGqG8gKApUvFJukc4VM4VAjaIoEnjfG0EuItWGDL_IeS69KeTacgnsBRut1QJtWemusB8U-9OlkKVkFJ3g-GGBrDuITQJHvZ1lF8WtpD4_9vRAfnwvdeHn_6moxl6vWjniwiN08e8a3CVd3KVFCbyKXvN7cp33JcA2_Gmt4Km_NyXOSwIFePCKw_8cdwxPx7PJuTz_PP16BM8iqg5Tiq9hy_mLfePSrKV-G8LqDvL0JMo |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB1BkRAXoNBCKFAfkIBDkl1nHDvHqrAqH11ViEp7s2zHESuoGzW7F349dpwsbFUuvUXxOJEzM_bEfvMG4I2wFaKmTRqqGadY-Std8CY1pq6ZmCotJiHf-XRenpzj5wVbDFsX3QCrdEYvM_frInPLHz22sr0w-YgTy89OjwtvNyxv6ya_C_e8x1I-_qZv2HZFzD2hfgpGiiNbIy_y1qkuC4tkyErtq_WFJGLKcGthitjEQHjqxW8KPq9jKP9ZlGaPYDEOJ2JRfmbrlc7M72tMj7cY72N4OASq5CgK7MId657A7jAVdOTdwFf9_ikU3-ZHZNkRRSIZbSDyIDbC0snSkSu7CvsWV-uOtOP79uB89vH78Uk61GJIDRN05bWI3Fo0JafWcM28W080V7WoAp8-U7yYqgoVoxPuv2bNFWWaTZqqMZpWvkexDzvu0tnnQBQiYzQUEhQlKl4rP1kXiplSoEZRlgm8HRUh20i5Ifujcl7IoA650V4Ce72eNmI9fVnln7DfC463KyErySj6hsObG2QzQG0SOBh1LQdv7iQNcXKgCQr9x1bvhuFsRTl7ue7klIuA1MX_S3AffHEfHiXwLFrO38ENFpgA27KpjUCgAN9u8ZbSU4H3lvHilv0O4f7Zh5n8-mn-5QAeRHAdphRfwo43F_vKR1sr_br3rD8tJCdK |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=RNA+is+a+structural+element+in+retrovirus+particles&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences+-+PNAS&rft.au=Muriaux%2C+D&rft.au=Mirro%2C+J&rft.au=Harvin%2C+D&rft.au=Rein%2C+A&rft.date=2001-04-24&rft.issn=0027-8424&rft.volume=98&rft.issue=9&rft.spage=5246&rft.epage=5251&rft_id=info:doi/10.1073%2Fpnas.091000398&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F98%2F9.cover.gif |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F98%2F9.cover.gif |