Colony blot assay: a useful method to detect multiple pneumococcal serotypes within clinical specimens
The efficacy of pneumococal conjugate vaccines in young children may be complicated by serotype replacement. We developed a colony blot assay which enables the identification of re-colonization with novel serotypes (replacement), overgrowth by minor co-colonizing serotypes or suppression of previous...
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Published in | FEMS immunology and medical microbiology Vol. 41; no. 3; pp. 259 - 264 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Elsevier B.V
01.07.2004
Blackwell Publishing Ltd Blackwell Oxford University Press |
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Abstract | The efficacy of pneumococal conjugate vaccines in young children may be complicated by serotype replacement. We developed a colony blot assay which enables the identification of re-colonization with novel serotypes (replacement), overgrowth by minor co-colonizing serotypes or suppression of previously predominant vaccine serotype strains as a result of vaccination. This method allows the identification of multiple serotypes in a single specimen in a ratio of 1:1000. In order to demonstrate the potential of our method, we investigated the consecutive nasopharyngeal samples of 26 children who had shown a shift in pneumococcal colonization after conjugate vaccination. Mixed colonization was found once in 15 pre-vaccination samples and four times in 26 post-vaccination samples. In the remaining children `true replacement' had presumably occurred. Hence, we conclude that the colony blot assay is an easy to apply method, which allows the identification of different pneumococcal serotypes within single clinical specimens. |
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AbstractList | The efficacy of pneumococal conjugate vaccines in young children may be complicated by serotype replacement. We developed a colony blot assay which enables the identification of re-colonization with novel serotypes (replacement), overgrowth by minor co-colonizing serotypes or suppression of previously predominant vaccine serotype strains as a result of vaccination. This method allows the identification of multiple serotypes in a single specimen in a ratio of 1:1000. In order to demonstrate the potential of our method, we investigated the consecutive nasopharyngeal samples of 26 children who had shown a shift in pneumococcal colonization after conjugate vaccination. Mixed colonization was found once in 15 pre-vaccination samples and four times in 26 post-vaccination samples. In the remaining children `true replacement' had presumably occurred. Hence, we conclude that the colony blot assay is an easy to apply method, which allows the identification of different pneumococcal serotypes within single clinical specimens. Abstract The efficacy of pneumococal conjugate vaccines in young children may be complicated by serotype replacement. We developed a colony blot assay which enables the identification of re-colonization with novel serotypes (replacement), overgrowth by minor co-colonizing serotypes or suppression of previously predominant vaccine serotype strains as a result of vaccination. This method allows the identification of multiple serotypes in a single specimen in a ratio of 1:1000. In order to demonstrate the potential of our method, we investigated the consecutive nasopharyngeal samples of 26 children who had shown a shift in pneumococcal colonization after conjugate vaccination. Mixed colonization was found once in 15 pre-vaccination samples and four times in 26 post-vaccination samples. In the remaining children ‘true replacement’ had presumably occurred. Hence, we conclude that the colony blot assay is an easy to apply method, which allows the identification of different pneumococcal serotypes within single clinical specimens. |
Author | Veenhoven, R.H Sanders, E.A.M Sluijter, M Hermans, P.W.M Bogaert, D de Groot, R |
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Cites_doi | 10.1097/00006454-200003000-00003 10.1128/JCM.33.6.1606-1612.1995 10.1086/315009 10.1016/S0140-6736(03)13772-5 10.1128/JCM.36.8.2248-2253.1998 10.1128/JCM.35.3.764-766.1997 10.1093/aje/154.1.85 10.1086/339525 10.3201/eid0503.990304 10.1056/NEJM200102083440602 10.1016/S0140-6736(00)02779-3 |
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Keywords | Replacement Unmasking Multiple serotypes Vaccination Streptococcus pneumoniae Streptococcaceae Immunology Microbiology Bacteria Micrococcales Method Serotype Detection |
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Snippet | The efficacy of pneumococal conjugate vaccines in young children may be complicated by serotype replacement. We developed a colony blot assay which enables the... Abstract The efficacy of pneumococal conjugate vaccines in young children may be complicated by serotype replacement. We developed a colony blot assay which... |
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SubjectTerms | Assaying Bacteriology Biological and medical sciences Carrier State - microbiology Child Child, Preschool Children Colonies Colonization Conjugates Culture Media Fundamental and applied biological sciences. Psychology Human papillomavirus Humans Immunization Immunoblotting - methods Infant Microbiology Miscellaneous Multiple serotypes Nasopharynx - microbiology Otitis Media - microbiology Otitis Media - prevention & control Pneumococcal Infections - microbiology Pneumococcal Infections - prevention & control Pneumococcal Vaccines - administration & dosage Replacement Serotypes Serotyping Streptococcus infections Streptococcus pneumoniae Streptococcus pneumoniae - classification Streptococcus pneumoniae - isolation & purification Unmasking Vaccination Vaccines Vaccines, Conjugate - administration & dosage |
Title | Colony blot assay: a useful method to detect multiple pneumococcal serotypes within clinical specimens |
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