The role of sucrose during maturation of black spruce (Picea mariana) and white spruce (Picea glauca) somatic embryos

• Published in: Physiologia plantarum Vol. 111; no. 3; pp. 381 - 388
• Main Authors: Iraqi, D, Tremblay, F.M. (Laval Univ., Ste-Foy (Canada))
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard International Publishers 01.03.2001; Blackwell
• Subjects: Biological and medical sciences; Biotechnology; EMBRION SOMATICO; EMBRYON SOMATIQUE; Eukaryotic cell cultures; FRUCTOSA; FRUCTOSE; Fundamental and applied biological sciences. Psychology; GLUCOSA; GLUCOSE; In vitro embryo culture; MADURACION; MATURATION; Methods. Procedures. Technologies; OSMOTIC PRESSURE; PICEA GLAUCA; PICEA MARIANA; Plant cells and fungal cells; PRESION OSMOTICA; PRESSION OSMOTIQUE; SACCHAROSE; SOMATIC EMBRYOS; SUCROSA; SUCROSE; Culture medium; Picea glauca; Sucrose; Starch; Interspecific comparison; Micropropagation; Concentration; Glucose; Fructose; Somatic embryo; Ripening; Softwood forest tree; Gymnospermae; Coniferales; Spermatophyta; Chemical composition; Osmotic pressure; Picea mariana; Protein content

The present study was conducted to understand the role of sucrose in the medium on the maturation of black spruce and white spruce somatic embryos. A maturation medium containing 6% sucrose, which hydrolyzed into glucose and fructose, gave significantly more embryos than a medium containing 3.16% of...