Postabsorptive hyperglucagonemia in patients with type 2 diabetes mellitus analyzed with a novel enzyme‐linked immunosorbent assay

• Published in: Journal of diabetes investigation Vol. 7; no. 3; pp. 324 - 331
• Main Authors: Matsuo, Toshihiro, Miyagawa, Jun‐ichiro, Kusunoki, Yoshiki, Miuchi, Masayuki, Ikawa, Takashi, Akagami, Takafumi, Tokuda, Masaru, Katsuno, Tomoyuki, Kushida, Akira, Inagaki, Takashi, Namba, Mitsuyoshi
• Format: Journal Article
• Language: English
• Published: Japan John Wiley & Sons, Inc 01.05.2016; John Wiley and Sons Inc
• Subjects: Adult; Aged; Antibodies, Monoclonal - administration & dosage; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - diagnosis; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzymes; Female; Glucagon; Glucagon (1–29); Glucagon - analysis; Glucagon - blood; Glucagon - immunology; Glucagon‐like peptides; Glucose; Glucose tolerance; Glucose Tolerance Test; Humans; Immunological tolerance; Insulin; Male; Middle Aged; Monoclonal antibodies; Original; Peptides; Plasma levels; Polyclonal antibodies; Proglucagon - analysis; Proglucagon - blood; Proglucagon - immunology; Radioimmunoassay; Studies; Type 2 diabetes mellitus; Young Adult; United States > US; Sweden; Japan; Type 2 diabetes mellitus; Glucagon (1–29); Glucagon‐like peptides

Aims/introduction The aims of the present study were to investigate the performance of a novel sandwich enzyme‐linked immunosorbent assay (ELISA) for measuring glucagon (1–29) with monoclonal antibodies against both the C‐ and N‐terminal regions of glucagon (1–29), and to analyze the differences in...