双向电泳分析普洱茶对小鼠肝脏MUP-1蛋白表达的影响
本文采用双向电泳技术,鉴定昆明鼠在饮用普洱茶后肝脏蛋白质组变化.结果表明,采用pH3～10胶条未达到理想的分离效果,在采用窄范围pH4～7胶条电泳后得到16个差异蛋白,其中15个蛋白经质谱鉴定得到蛋白的信息,主要尿蛋白（Major urinary protein,MUP-1）表达显著上调,该蛋白可以作为能量代谢的生物标志物,并在近年来被证明具有调节代谢的作用.饮用普洱茶后小鼠肝脏中MUP-1上调,说明普洱茶在小鼠糖代谢中具有促进作用,这些结果从另一个角度解释过去研究中普洱茶保健作用的机理....
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| Published in | 西南农业学报 Vol. 27; no. 1; pp. 428 - 432 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
云南农业大学,普洱茶学教育部重点实验室,云南昆明650201%吉林大学,生命科学学院,吉林长春130012
2014
普洱茶研究院,云南普洱665000%云南农业大学,普洱茶学教育部重点实验室,云南昆明650201%云南大学校长办公室,云南昆明,650228 吉林大学,生命科学学院,吉林长春130012 |
| Subjects | |
| Online Access | Get full text |
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| Summary: | 本文采用双向电泳技术,鉴定昆明鼠在饮用普洱茶后肝脏蛋白质组变化.结果表明,采用pH3～10胶条未达到理想的分离效果,在采用窄范围pH4～7胶条电泳后得到16个差异蛋白,其中15个蛋白经质谱鉴定得到蛋白的信息,主要尿蛋白（Major urinary protein,MUP-1）表达显著上调,该蛋白可以作为能量代谢的生物标志物,并在近年来被证明具有调节代谢的作用.饮用普洱茶后小鼠肝脏中MUP-1上调,说明普洱茶在小鼠糖代谢中具有促进作用,这些结果从另一个角度解释过去研究中普洱茶保健作用的机理. |
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| Bibliography: | 51-1213/S SONG Shuang,YAN Liang,WANG Xuan-jun,HAO Shu-mei,SHENG Jun（ 1.College of Life Sciences, Jilin University, Jilin Changchun 130012, China;Key Laboratory of Puer Tea Science, Ministry of Education, Yunnan Agriculture University, Yunnan Kunming 650201, China; 2.College of Life Sciences, Jilin University, Jilin Changchun 130012, China;Pu＇er Institute of Pu-er Tea,Yunnan Puer 665000 ,China; 3.Yunnan University, Yunnan Kunming 650091, China;） 2D-Electrophoresis（2DE） ;Pu-er-tea; Major urinary protein（MUP-1） ;Glucose metabolism In this paper,2D-Electrophoresis was employed to analyze the differential expression of mouse liver proteome.The result showed that slight difference was detected with 3-10 strips,sixteen difference expression proteins were detected with pH 4-7 strips,and 15 protein ID were verified with ESI-MS-MS succeesfully,of which major urinary protein（MUP-1） was one of up regulated protein,which served as the biomarker of energy metabolism,testifying the regulating role of metabolism in recent years |
| ISSN: | 1001-4829 |