Determination of Delta-aminolevulinic Acid in Plasma using High-performance Liquid Chromatography: A Sensitive Indicator of Lead Effects
We developed a simple and reliable method for determining delta-aminolevulinic acid (ALA) in plasma (ALA-P) by fluorometric high-performance liquid chromatography. The method was applicable to 100 μl of plasma and the mean ± standard deviation for analytical recovery was 100.4 ± 2.6% when 50 pg/1 of...
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Published in | Industrial Health Vol. 32; no. 2; pp. 85 - 96 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
National Institute of Occupational Safety and Health
1994
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Subjects | |
Online Access | Get full text |
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Summary: | We developed a simple and reliable method for determining delta-aminolevulinic acid (ALA) in plasma (ALA-P) by fluorometric high-performance liquid chromatography. The method was applicable to 100 μl of plasma and the mean ± standard deviation for analytical recovery was 100.4 ± 2.6% when 50 pg/1 of ALA was added to 14 plasma samples. The detection limit for ALA-P was 2.0 μg/l (signal-to-noise ratio was 5) and the standard curve was linear in a wide range up to 400 μg/l. The ALA-P level for 26 unexposed male adults was 8.6 ± 1.3 μg/l. In 72 male lead workers, logarithm of ALA-P concentration was significantly correlated with blood lead concentrations (Pb-B) of 2.5-115.4 μg/dl (r = 0.924, p < 0.001). Even at the Pb-B level less than 15 pg/dl, significant linear relationships were found between Pb-B and log ALA-P (r = 0.632, p < 0.01), between Pb-B and delta-aminolevulinic acid dehydratase activity (ALA-D) (r =-0.573, p < 0.05), and between ALA-D and ALA-P (r =-0.765, p < 0.001). It is suggested that ALA-P determined by the present method is a useful indicator of biological effects of lead, es-pecially in the wide range of Pb-B level. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0019-8366 1880-8026 |
DOI: | 10.2486/indhealth.32.85 |