DNA Conformation Induces Adaptable Binding by Tandem Zinc Finger Proteins

• Published in: Cell Vol. 173; no. 1; pp. 221 - 233.e12
• Main Authors: Patel, Anamika, Yang, Peng, Tinkham, Matthew, Pradhan, Mihika, Sun, Ming-An, Wang, Yixuan, Hoang, Don, Wolf, Gernot, Horton, John R., Zhang, Xing, Macfarlan, Todd, Cheng, Xiaodong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.03.2018; Elsevier
• Subjects: Amino Acid Sequence; Animals; AT-rich; Base Sequence; BASIC BIOLOGICAL SCIENCES; binding capacity; Binding Sites; C2H2 zinc fingers; Carrier Proteins - chemistry; Carrier Proteins - classification; Carrier Proteins - genetics; Carrier Proteins - metabolism; DNA; DNA - chemistry; DNA - metabolism; DNA conformation; evolution; Humans; Igf2; imprinting; insulin-like growth factor II; Insulin-Like Growth Factor II - chemistry; Insulin-Like Growth Factor II - genetics; Insulin-Like Growth Factor II - metabolism; KRAB; Mice; Molecular Dynamics Simulation; mutation; Nuclear Proteins - chemistry; Nuclear Proteins - classification; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nucleic Acid Conformation; Pan troglodytes; Phylogeny; Polymorphism, Single Nucleotide; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Sequence Alignment; shortened minor groove; thymine; transcription factors; Zfp568; zinc finger motif; imprinting; Zfp568; C2H2 zinc fingers; DNA conformation; Igf2; shortened minor groove; KRAB; AT-rich

Tandem zinc finger (ZF) proteins are the largest and most rapidly diverging family of DNA-binding transcription regulators in mammals. ZFP568 represses a transcript of placental-specific insulin like growth factor 2 (Igf2-P0) in mice. ZFP568 binds a 24-base pair sequence-specific element upstream of...