Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing

• Published in: Plant biotechnology journal Vol. 17; no. 8; pp. 1623 - 1635
• Main Authors: Zhang, Zhengzhi, Hua, Lei, Gupta, Ajay, Tricoli, David, Edwards, Keith J., Yang, Bing, Li, Wanlong
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.08.2019; John Wiley and Sons Inc
• Subjects: Agrobacterium; Agrobacterium mediation; biolistics; biotechnology; Cereal crops; corn; CRISPR; CRISPR-Cas Systems; CRISPR/Cas9; Deoxyribonucleic acid; DNA; DNA binding proteins; Editing; Efficiency; Enzymes; Gene deletion; Gene Editing; Gene silencing; Genes; Genetic testing; Genetic transformation; Genome editing; Genome, Plant; Genomes; Genomics; Grain; grain regulation. mutations; homozygosity; Mutation; Nucleases; Plant populations; Plant species; Plants, Genetically Modified; plasmids; Promoter Regions, Genetic; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - genetics; Sequence Deletion; spikelets; Target detection; Transgenic plants; Triticum - genetics; Ubiquitin; Wheat; Zinc finger proteins; genome editing; wheat; Agrobacterium mediation; CRISPR/Cas9; grain regulation. mutations

Summary CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA delivery methods. In wheat, the biolistics base...