Lack of synchrony among multiple nuclei induces partial DNA fragmentation in V79 cells polyploidized by demecolcine
. The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidize...
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| Published in | Cell proliferation Vol. 32; no. 6; pp. 337 - 349 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.12.1999
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| Abstract | . The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidized by demecolcine but not by K‐252a, which produced mononuclear cells. A lack of synchrony in the nuclear cycle was observed among nuclei in multinuclear polyploidized cells. Partial DNA fragmentation, defined as DNA fragmentation of a nucleus in a multinuclear cell, was detected using the TUNEL method in V79 cells polyploidized by demecolcine but not by K‐252a. Apoptosis occurred earlier in cell populations treated with demecolcine than in these treated with K‐252a once the drugs were removed from the medium, suggesting that polyploidized cells with separate nuclei tend to apoptose earlier than those with mononuclei. |
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| AbstractList | Abstract.
The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidized by demecolcine but not by K‐252a, which produced mononuclear cells. A lack of synchrony in the nuclear cycle was observed among nuclei in multinuclear polyploidized cells. Partial DNA fragmentation, defined as DNA fragmentation of a nucleus in a multinuclear cell, was detected using the TUNEL method in V79 cells polyploidized by demecolcine but not by K‐252a. Apoptosis occurred earlier in cell populations treated with demecolcine than in these treated with K‐252a once the drugs were removed from the medium, suggesting that polyploidized cells with separate nuclei tend to apoptose earlier than those with mononuclei. The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K-252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidized by demecolcine but not by K-252a, which produced mononuclear cells. A lack of synchrony in the nuclear cycle was observed among nuclei in multinuclear polyploidized cells. Partial DNA fragmentation, defined as DNA fragmentation of a nucleus in a multinuclear cell, was detected using the TUNEL method in V79 cells polyploidized by demecolcine but not by K-252a. Apoptosis occurred earlier in cell populations treated with demecolcine than in these treated with K-252a once the drugs were removed from the medium, suggesting that polyploidized cells with separate nuclei tend to apoptose earlier than those with mononuclei. . The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre formation and protein kinases, respectively. A variety of nuclear morphologies, including multinuclei, were observed in V79 cells polyploidized by demecolcine but not by K‐252a, which produced mononuclear cells. A lack of synchrony in the nuclear cycle was observed among nuclei in multinuclear polyploidized cells. Partial DNA fragmentation, defined as DNA fragmentation of a nucleus in a multinuclear cell, was detected using the TUNEL method in V79 cells polyploidized by demecolcine but not by K‐252a. Apoptosis occurred earlier in cell populations treated with demecolcine than in these treated with K‐252a once the drugs were removed from the medium, suggesting that polyploidized cells with separate nuclei tend to apoptose earlier than those with mononuclei. |
| Author | Ohdoi, C. Fujikawa-Yamamoto, K. Zong, Z. -p. Murakami, M. Yamagishi, H. Yamaguchi, N. |
| AuthorAffiliation | 1 Division of Basic Science, Research Institute of Medical Science 2 †Department of Serology, Kanazawa Medical University, Uchinada, Ishikawa, Japan |
| AuthorAffiliation_xml | – name: 1 Division of Basic Science, Research Institute of Medical Science – name: 2 †Department of Serology, Kanazawa Medical University, Uchinada, Ishikawa, Japan |
| Author_xml | – sequence: 1 givenname: K. surname: Fujikawa-Yamamoto fullname: Fujikawa-Yamamoto, K. email: Japan.fujikawa@kanazawa-med.ac.jp organization: Division of Basic Science, Research Institute of Medical Science – sequence: 2 givenname: C. surname: Ohdoi fullname: Ohdoi, C. organization: Division of Basic Science, Research Institute of Medical Science – sequence: 3 givenname: H. surname: Yamagishi fullname: Yamagishi, H. organization: Division of Basic Science, Research Institute of Medical Science – sequence: 4 givenname: Z. -p. surname: Zong fullname: Zong, Z. -p. organization: Division of Basic Science, Research Institute of Medical Science – sequence: 5 givenname: M. surname: Murakami fullname: Murakami, M. organization: Division of Basic Science, Research Institute of Medical Science – sequence: 6 givenname: N. surname: Yamaguchi fullname: Yamaguchi, N. organization: †Department of Serology, Kanazawa Medical University, Uchinada, Ishikawa, Japan |
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| Cites_doi | 10.1016/0955-0674(92)90060-P 10.1016/0014-4827(90)90129-X 10.1002/j.1460-2075.1991.tb07673.x 10.1247/csf.18.449 10.1083/jcb.91.3.131s 10.1038/225159a0 10.1083/jcb.111.3.1047 10.1002/jss.400090402 10.1242/jcs.10.2.495 10.1006/bbrc.1994.2728 10.1073/pnas.87.24.9553 10.1007/BF00685842 10.1247/csf.22.399 10.1016/0955-0674(93)90002-8 10.1083/jcb.107.4.1531 10.1083/jcb.126.6.1341 10.1083/jcb.122.2.295 10.1038/332561a0 10.1247/csf.19.391 10.1083/jcb.115.5.1275 10.1083/jcb.99.3.1066 10.1002/path.1711050103 10.1016/S0074-7696(08)62312-8 10.1083/jcb.66.1.188 10.1016/S0955-0674(05)80014-6 10.1016/0006-291X(87)90293-2 10.1002/jcp.1041580320 10.1016/S0092-8674(85)80099-4 10.1002/ijc.2910090307 |
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| Snippet | . The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre... The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K-252a, inhibitors of spindle fibre... The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle fibre... Abstract. The nuclear morphology of polyploidized cells was examined in V79 Chinese hamster cells polyploidized by demecolcine or K‐252a, inhibitors of spindle... |
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| SubjectTerms | Animals Cell Line Cell Nucleus - drug effects Cell Nucleus - ultrastructure Chromosomes Cricetinae Cricetulus Demecolcine - pharmacology DNA Fragmentation Flow Cytometry Original Polyploidy |
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| Title | Lack of synchrony among multiple nuclei induces partial DNA fragmentation in V79 cells polyploidized by demecolcine |
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