鳜肌酸激酶M-CK cDNA的克隆与组织表达分析
利用RT-PCR和cDNA未端快速扩增法（RACE）克隆了鳜（Siniperca chuatsi）肌酸激酶（creatine kinase，CK）cDNA序列，并分析了该基因的结构特征和系统关系。鳜CK cDNA序列全长1586bp，包括5′端非翻译区92bp，3′端非翻译区348bp和开放阅读框（ORF）1146bp，共编码381个氨基酸。鳜CK具有脊椎动物CK共有的保守结构域和肌型肌酸激酶（M—CK）同工酶的特异识别位点；氨基酸序列与M-CK型的相似度最高，而与脑型肌酸激酶（B—CK）和线粒体型肌酸激酶（Mi—CKs）的相似度较低；在CK系统关系树中鳜CK与M—CK群聚类。这些均表明，鳜C...
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| Published in | Dōngwùxué yánjiū Vol. 31; no. 1; pp. 77 - 83 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
农业部,水产种质资源与利用重点开放实验室,上海海洋大学,上海,201306
2010
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0254-5853 |
| DOI | 10.3724/SP.J.1141.2010.01077 |
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| Abstract | 利用RT-PCR和cDNA未端快速扩增法（RACE）克隆了鳜（Siniperca chuatsi）肌酸激酶（creatine kinase，CK）cDNA序列，并分析了该基因的结构特征和系统关系。鳜CK cDNA序列全长1586bp，包括5′端非翻译区92bp，3′端非翻译区348bp和开放阅读框（ORF）1146bp，共编码381个氨基酸。鳜CK具有脊椎动物CK共有的保守结构域和肌型肌酸激酶（M—CK）同工酶的特异识别位点；氨基酸序列与M-CK型的相似度最高，而与脑型肌酸激酶（B—CK）和线粒体型肌酸激酶（Mi—CKs）的相似度较低；在CK系统关系树中鳜CK与M—CK群聚类。这些均表明，鳜CK属脊椎动物M-CK型。RT-PCR分析表明，鳜M—CK存成体不同组织中的表达量小同，其中，在皮肤、卵巢、肾脏、胃、肌肉和心脏中表达较强；而在眼和脑、肝胰脏中表达较弱。 |
|---|---|
| AbstractList | 利用RT-PCR和cDNA未端快速扩增法（RACE）克隆了鳜（Siniperca chuatsi）肌酸激酶（creatine kinase，CK）cDNA序列，并分析了该基因的结构特征和系统关系。鳜CK cDNA序列全长1586bp，包括5′端非翻译区92bp，3′端非翻译区348bp和开放阅读框（ORF）1146bp，共编码381个氨基酸。鳜CK具有脊椎动物CK共有的保守结构域和肌型肌酸激酶（M—CK）同工酶的特异识别位点；氨基酸序列与M-CK型的相似度最高，而与脑型肌酸激酶（B—CK）和线粒体型肌酸激酶（Mi—CKs）的相似度较低；在CK系统关系树中鳜CK与M—CK群聚类。这些均表明，鳜CK属脊椎动物M-CK型。RT-PCR分析表明，鳜M—CK存成体不同组织中的表达量小同，其中，在皮肤、卵巢、肾脏、胃、肌肉和心脏中表达较强；而在眼和脑、肝胰脏中表达较弱。 Q959.483.09%Q786; 利用RT-PCR和cDNA末端快速扩增法(RACE)克隆了鳜(Siniperca chuatsi)肌酸激酶(creatine kinase,CK)cDNA序列,并分析了该基因的结构特征和系统关系.鳜CK cDNA序列全长1586 bp,包括5'端非翻译区92 bp,3'端非翻译区348 bp和开放阅读框(ORF)1146 bp,共编码381个氨基酸.鳜CK具有脊椎动物CK 共有的保守结构域和肌型肌酸激酶(M-CK)同工酶的特异识别位点;氨基酸序列与M-CK型的相似度最高,而与脑型肌酸激酶(B-CK)和线粒体型肌酸激酶(Mi-CKs)的相似度较低;在CK系统关系树中鳜CK与M-CK群聚类.这些均表明,鳜CK属脊椎动物M-CK型.RT-PCR分析表明,鳜M-CK在成体不同组织中的表达量不同,其中,在皮肤、卵巢、肾脏、胃、肌肉和心脏中表达较强;而在眼和脑、肝胰脏中表达较弱. |
| Abstract_FL | The ereatine kinase (CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of eDNA ends (RACE) methods. The structural characteristics and phylogeny of this gene were analyzed. Sequence analysis revealed a 1 586 bp eDNA sequence containing 92 bp 5'-untranslated region, 348 bp 3'-untranslated region and 1146 bp open reading frame (ORF), which encoded 381 amino acids. Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK (M-CK) isozyme were identified in S. chuatsi CK. Siniperca chuatsi CK showed a higher similarity with vertebrates M-CK isozyme than other CK isozymes (Brain CK, Mitochondrial CKs) and grouped with M-CK isozyme in CK phylogeny, which strongly supported that S. chuatsi CK belongs to M-CK isozyme type. Semi-quantitative RT-PCR analysis demonstrated that the M-CK transcript expression varied among the different tissues and was detected at a high level in skin, ovary, kidney, stomach, muscle and heart, but lower in eye, brain and liver. |
| Author | 张敏 赵金良 邓燕飞 |
| AuthorAffiliation | 农业部水产种质资源与利用重点开放实验室,上海海洋大学,上海201306 |
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| Author_FL | ZHANG Min DENG Yan-Fei ZHAO Jin-Liang |
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| DOI | 10.3724/SP.J.1141.2010.01077 |
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| Keywords | Siniperca chuatsi 组织表达 Creatine kinase 鳜 肌酸激酶 cDNA末端快速扩增 Rapid amplification of cDNA ends Tissue expression |
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| Snippet | 利用RT-PCR和cDNA未端快速扩增法（RACE）克隆了鳜（Siniperca chuatsi）肌酸激酶（creatine kinase，CK）cDNA序列，并分析了该基因的结构特征和系统关系。鳜CK... Q959.483.09%Q786; 利用RT-PCR和cDNA末端快速扩增法(RACE)克隆了鳜(Siniperca chuatsi)肌酸激酶(creatine kinase,CK)cDNA序列,并分析了该基因的结构特征和系统关系.鳜CK cDNA序列全长1586 bp,包括5'端非翻译区92... |
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| SubjectTerms | cDNA末端快速扩增 组织表达 肌酸激酶 鳜 |
| Title | 鳜肌酸激酶M-CK cDNA的克隆与组织表达分析 |
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