碱性蛋白酶酶解波纹巴非蛤制备小分子肽

以波纹巴非蛤为原料，用碱性蛋白酶酶解波纹巴非蛤肉蛋白制备小分子肽，并运用响应面方法设计优化波纹巴非蛤酶解的工艺条件。结果表明：在pH值6．5、液固比为3：1、酶含量为2．50％、酶解温度为50．4℃、酶解时间122．60min的条件下，小分子肽的提取率最高、为63．04％。...

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Bibliographic Details
Published in广东农业科学 Vol. 38; no. 19; pp. 1 - 5
Main Author 袁毅桦 孙恢礼 陈晓刚 陈纯馨 林凯生 戎海武
Format Journal Article
LanguageChinese
Published 佛山科学技术学院化学与化工系,广东佛山,528000%中国科学院南海海洋研究所,广东广州,510301%佛山科学技术学院信息科学与数学系,广东佛山,528000 2011
Subjects
响应面
小分子肽
波纹巴非蛤
酶解
响应面
波纹巴非蛤
小分子肽
酶解
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ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2011.19.001

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Summary:以波纹巴非蛤为原料，用碱性蛋白酶酶解波纹巴非蛤肉蛋白制备小分子肽，并运用响应面方法设计优化波纹巴非蛤酶解的工艺条件。结果表明：在pH值6．5、液固比为3：1、酶含量为2．50％、酶解温度为50．4℃、酶解时间122．60min的条件下，小分子肽的提取率最高、为63．04％。
Bibliography:Paphia undulata; enzymolysis; response surface; micromolecule peptide
44-1267/S
YUAN Yi-hua, SUN Hui-li, CHEN Xiao-gang ,CHEN Chun-xin, LIN Kai-sheng, RONG Hai-wu （1. Department of Chemistry and Chemical Engineering, Foshan University, Foshan 528000, China; 2. South China Sea Institute of Oceanlogy, Chinese Academy of Sciences, Guangzhou 510301, China; 3. Department of Information Sciences and Mathematics, Foshan University, Foshan 528000, China）
With Paphia undulate as the raw material, the micromolecule peptide was prepared by the enzymolysis of Paphia undulate meat with alkaline protease. Moreover, the optimal conditions for enzymolysis of Paphia undulate were designed with response surface method. The resuhs showed that the optimal conditions were as follows： when the pH was 6.5, liquid-to-solid ratio was 3：1, enzyme loading was 2.50%, enzymolysis temperature was 50.4℃, enzymolysis time was 122.60 min, the extraction rate of micromolecule peptide was the highest, reached 63.04%.
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2011.19.001