Polymorphisms in Phytophthora infestans: four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions

• Published in: Applied and Environmental Microbiology Vol. 64; no. 10; pp. 4007 - 4014
• Main Authors: Griffith, G.W. (University of Wales, Wales, UK.), Shaw, D.S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1998
• Subjects: ADN; Biological and medical sciences; DNA; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; LYCOPERSICON ESCULENTUM; Microbiology; MITOCHONDRIA; MITOCHONDRIE; MITOCONDRIA; Mycology; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology; PCR; Phytopathology. Animal pests. Plant and forest protection; PHYTOPHTHORA INFESTANS; POLYMERASE CHAIN REACTION; RFLP; SOLANUM TUBEROSUM; Variation, races, biotypes, parasitic specialization, genetics; Plant pathogen; Solanum tuberosum; Phycomycetes; Primer; Mitochondrial DNA; Identification; Method; Fungi; Polymerase chain reaction; Restriction fragment length polymorphism; Dicotyledones; Angiospermae; Lycopersicon esculentum; Phytophthora infestans; Spermatophyta; Solanaceae; Haplotype; Thallophyta

Four pairs of primers were designed for PCR amplification of known polymorphic regions of the mitochondrial genome of Phytophthora infestans. Digestion of the amplified products with restriction enzymes allows identification of previously identified haplotypes. Product P2 cut with MspI uniquely iden...