High-throughput evaluation of hemolytic activity through precise measurement of colony and hemolytic zone sizes of engineered Bacillus subtilis on blood agar

• Published in: Biology Methods and Protocols Vol. 9; no. 1; p. bpae044
• Main Authors: Bamba, Takahiro, Aoki, Rina, Hori, Yoshimi, Ishikawa, Shu, Yoshida, Ken-ichi, Taoka, Naoaki, Kobayashi, Shingo, Yasueda, Hisashi, Kondo, Akihiko, Hasunuma, Tomohisa
• Format: Journal Article
• Language: English
• Published: England Oxford University Press (OUP) 2024; Oxford University Press
• Subjects: Methods; Methods Article; surfactin; mycosbutilin; biosurfactant; blood agar lysis method; Bacillus subtilis

Biosurfactants have remarkable characteristics, such as environmental friendliness, high safety, and excellent biodegradability. Surfactin is one of the best-known biosurfactants produced by Bacillus subtilis. Because the biosynthetic pathways of biosurfactants, such as surfactin, are complex, mutag...