Isotachophoresis for fractionation and recovery of cytoplasmic RNA and nucleus from single cells

• Published in: Electrophoresis Vol. 36; no. 14; pp. 1658 - 1662
• Main Authors: Kuriyama, Kentaro, Shintaku, Hirofumi, Santiago, Juan G.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.07.2015
• Subjects: Cell Nucleus - chemistry; Cytoplasm - chemistry; Deoxyribonucleic acid; DNA; DNA - isolation & purification; Electrodes; Electrophoresis; Equipment Design; Fractionation; Gene expression; Isotachophoresis - instrumentation; Lab-On-A-Chip Devices; Microfluidics; Nuclei; Ribonucleic acids; RNA; RNA - isolation & purification; Single cell analysis; Single-Cell Analysis - instrumentation; Electrophoresis; RNA; DNA; Microfluidics; Single cell analysis

There is a substantial need for simultaneous analyses of RNA and DNA from individual single cells. Such analysis provides unique evidence of cell‐to‐cell differences and the correlation between gene expression and genomic mutation in highly heterogeneous cell populations. We present a novel microflu...