Spatiotemporal ablation of myelinating glia-specific neurofascin (NfascNF155) in mice reveals gradual loss of paranodal axoglial junctions and concomitant disorganization of axonal domains

• Published in: Journal of neuroscience research Vol. 87; no. 8; pp. 1773 - 1793
• Main Authors: Pillai, Anilkumar M., Thaxton, Courtney, Pribisko, Alaine L., Cheng, Jr-Gang, Dupree, Jeffrey L., Bhat, Manzoor A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2009
• Subjects: Animals; axoglial junctions; axonal domains; Axons - metabolism; Axons - pathology; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Demyelinating Diseases - genetics; Demyelinating Diseases - pathology; Demyelinating Diseases - physiopathology; Disease Models, Animal; Mice; Mice, Knockout; Mice, Mutant Strains; Mice, Transgenic; Movement Disorders - genetics; Movement Disorders - pathology; Movement Disorders - physiopathology; myelin; Myelin Proteolipid Protein - genetics; Myelin Proteolipid Protein - metabolism; Myelin Sheath - metabolism; Myelin Sheath - pathology; myelinated axons; Nerve Fibers, Myelinated - metabolism; Nerve Fibers, Myelinated - pathology; Nerve Growth Factors - genetics; Nerve Growth Factors - metabolism; Neural Conduction - genetics; Neuroglia - metabolism; Neuroglia - pathology; paranodes; Peripheral Nerves - metabolism; Peripheral Nerves - pathology; Peripheral Nerves - physiopathology; Ranvier's Nodes - metabolism; Ranvier's Nodes - pathology; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Wallerian Degeneration - genetics; Wallerian Degeneration - pathology; Wallerian Degeneration - physiopathology
• ISSN: 0360-4012; 1097-4547; 1097-4547
• DOI: 10.1002/jnr.22015

The evolutionary demand for rapid nerve impulse conduction led to the process of myelination‐dependent organization of axons into distinct molecular domains. These domains include the node of Ranvier flanked by highly specialized paranodal domains where myelin loops and axolemma orchestrate the axoglial septate junctions. These junctions are formed by interactions between a glial isoform of neurofascin (NfascNF155) and axonal Caspr and Cont. Here we report the generation of myelinating glia‐specific NfascNF155 null mouse mutants. These mice exhibit severe ataxia, motor paresis, and death before the third postnatal week. In the absence of glial NfascNF155, paranodal axoglial junctions fail to form, axonal domains fail to segregate, and myelinated axons undergo degeneration. Electrophysiological measurements of peripheral nerves from NfascNF155 mutants revealed dramatic reductions in nerve conduction velocities. By using inducible PLP‐CreER recombinase to ablate NfascNF155 in adult myelinating glia, we demonstrate that paranodal axoglial junctions disorganize gradually as the levels of NfascNF155 protein at the paranodes begin to drop. This coincides with the loss of the paranodal region and concomitant disorganization of the axonal domains. Our results provide the first direct evidence that the maintenance of axonal domains requires the fence function of the paranodal axoglial junctions. Together, our studies establish a central role for paranodal axoglial junctions in both the organization and the maintenance of axonal domains in myelinated axons. © 2009 Wiley‐Liss, Inc.