Anticancer and antifungal efficiencies of purified chitinase produced from Trichoderma viride under submerged fermentation

• Published in: Journal of general and applied microbiology Vol. 66; no. 1; pp. 32 - 40
• Main Authors: Isaac, George Saad, Abu-Tahon, Medhat Ahmed
• Format: Journal Article
• Language: English
• Published: Japan Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 01.01.2020; Japan Science and Technology Agency
• Subjects: anticancer efficiency; Antifungal activity; Biocompatibility; Body weight; Calcium; Calcium ions; Carbon dioxide; Cell lines; Chitin; Chitinase; Cobalt; Colloid chitin; Copper; Enzymes; Ethylenediaminetetraacetic acids; Fermentation; Fungi; Fungicides; Fusarium oxysporum; Lethal dose; Maltose; Mercury (metal); pH effects; Protected areas; Protein folding; Protein purification; Proteins; purification; submerged fermentation; Supplements; Tomatoes; Trichoderma viride; Wilt; Yeast; Zinc; submerged fermentation; chitinase; anticancer efficiency; antifungal activity; Trichoderma viride; purification

Trichoderma viride AUMC 13021 isolated from Mangrove soil of Ras Mohammed protected area at Sharm El-Sheikh, Egypt, was optimized to promote chitinase activity under submerged fermentation. The maximum enzyme yield (38.33 U/mg protein) was obtained at 1.4% of colloidal chitin, 96 h of incubation, 35...