Transcranial Imaging of Functional Cerebral Hemodynamic Changes in Single Blood Vessels using in vivo Photoacoustic Microscopy

• Published in: Journal of cerebral blood flow and metabolism Vol. 32; no. 6; pp. 938 - 951
• Main Authors: Liao, Lun-De, Lin, Chin-Teng, Shih, Yen-Yu I, Duong, Timothy Q, Lai, Hsin-Yi, Wang, Po-Hsun, Wu, Robby, Tsang, Siny, Chang, Jyh-Yeong, Li, Meng-Lin, Chen, You-Yin
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.06.2012; Nature Publishing Group; Sage Publications Ltd
• Subjects: Animals; Arteries; Arterioles; Biological and medical sciences; Blood vessels; Brain; Cerebral Arteries - physiology; Cerebral blood flow; Cerebrovascular Circulation - physiology; Cortex; Electrical stimuli; Hemodynamics; Hemodynamics - physiology; Hemoglobin; Hemoglobins - metabolism; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Mice; Microscopy; Nervous system; Neuroimaging; Neurology; Oxygen; Oxygen - metabolism; Photoacoustic Techniques - instrumentation; Photoacoustic Techniques - methods; Photoacoustics; Probes; Skull; spatial discrimination; superior sagittal sinus; Ultrasonic investigative techniques; Vascular diseases and vascular malformations of the nervous system; Veins; transcranial photoacoustic microscopy; hemodynamic response; hemoglobin oxygen saturation; cerebral blood volume; forepaw electrical stimulation; total hemoglobin concentration; Optoacoustical microscopy; Oxygen; Nervous system diseases; Saturation; Cerebral disorder; Encephalon; Blood volume; Central nervous system disease; Hemoglobin; Hemodynamics; Cerebrovascular disease; Functional imaging

Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel f...