Chemically inactivated adenoviral vectors that can efficiently transduce target cells when delivered in the form of virus-microbead conjugates

• Published in: Gene therapy Vol. 12; no. 6; pp. 521 - 533
• Main Authors: Pandori, M W, Sano, T
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2005; Nature Publishing Group
• Subjects: Adenoviridae - genetics; Adenovirus; Adenoviruses; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Antibodies, Monoclonal - pharmacology; Applied cell therapy and gene therapy; Avidin - pharmacology; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Biotechnology; Biotinylation; Cell Biology; Cell Line, Tumor; Cell lines; Coxsackie and Adenovirus Receptor-Like Membrane Protein; Design and construction; Dogs; Expression vectors; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene targeting; Gene Therapy; Genetic Therapy - methods; Genetic vectors; Genetic Vectors - administration & dosage; Genetic Vectors - genetics; Health. Pharmaceutical industry; Human Genetics; Humans; Indicators and Reagents - administration & dosage; Industrial applications and implications. Economical aspects; Infectivity; Integrin alphaV - immunology; Medical sciences; Methods; Mice; Microspheres; Nanotechnology; Neoplasms - therapy; Physiological aspects; Polymerase Chain Reaction - methods; Rats; Receptors, Virus - immunology; Receptors, Virus - metabolism; research-article; Streptavidin - administration & dosage; Transduction, Genetic - methods; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Virus Inactivation; United States; gene delivery; biotinylation; virus targeting; microbeads (nanocarriers); lectin; adenovirus; Virus; Lectin; Adenoviridae; Targeting; Target cell; Gene therapy; Vector
• ISSN: 0969-7128; 1476-5462
• DOI: 10.1038/sj.gt.3302420

Safe and effective use of viral vectors for gene therapeutics requires versatile control over their delivery to target sites in human subjects. We have developed a strategy for the creation of adenoviral vectors that possess conditional infectivity. The adenoviral vectors used were inactivated chemi...