Establishment of a simple prediction method for DNA melting temperature: high-resolution melting curve analysis of PCR products

• Published in: PloS one Vol. 20; no. 4; p. e0321885
• Main Authors: Zhou, Yunchao, Ha, Shan, Xu, Yuzhao, Qin, Xiaoshi, Ma, Yixin, Lu, Jianghuan, Wang, Bo, Cai, Jie, Duan, Zhiao, Cong, Bin, Chen, Jianhua, Deng, Jianqiang
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.04.2025
• Subjects: Accuracy; Base Composition; Base pairs; Biology and Life Sciences; Chemical analysis; Computer and Information Sciences; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - genetics; Enthalpy; Entropy; Evaluation; Gene sequencing; High resolution; Laboratories; Marine microorganisms; Melt temperature; Melting; Melting curve; Nearest-neighbor; Nucleic Acid Denaturation; Nucleotide sequence; Physical Sciences; Plankton; Polymerase chain reaction; Polymerase Chain Reaction - methods; Predictions; Real time; Research and Analysis Methods; Seawater; Temperature; Thermal properties; Thermal stability; Thermodynamics; Transition Temperature; Water analysis; China

High-resolution melting analysis is a technique that leverages the principle that the thermal stability of dsDNA is influenced by its length and base composition. This method generates a melting curve by real-time monitoring of the changes in fluorescence signal as dsDNA melts during the heating pro...