Establishment of a simple prediction method for DNA melting temperature: high-resolution melting curve analysis of PCR products - Zhou, Yunchao; Ha, Shan; Xu, Yuzhao; Qin, Xiaoshi; Ma, Yixin; Lu, Jianghuan; Wang, Bo; Cai, Jie; Duan, Zhiao; Cong, Bin; Chen, Jianhua; Deng, Jianqiang (PloS one, 2025)
Keywords: Accuracy; Base Composition; Base pairs; Biology and Life Sciences; Chemical analysis; Computer and Information Sciences; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - genetics; Enthalpy; Entropy; Evaluation; Gene sequencing; High resolution; Laboratories; Marine microorganisms; Melt temperature; Melting; Melting curve; Nearest-neighbor; Nucleic Acid Denaturation; Nucleotide sequence; Physical Sciences; Plankton; Polymerase chain reaction; Polymerase Chain Reaction - methods; Predictions; Real time; Research and Analysis Methods; Seawater; Temperature; Thermal properties; Thermal stability; Thermodynamics; Transition Temperature; Water analysis; China