intrinsically disordered C terminus allows the La protein to assist the biogenesis of diverse noncoding RNA precursors

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 4; pp. 1308 - 1313
• Main Authors: Kucera, Nathan J, Hodsdon, Michael E, Wolin, Sandra L
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 25.01.2011; National Acad Sciences
• Subjects: Amino Acid Sequence; Anticodon; Anticodon - genetics; Anticodon - metabolism; binding capacity; Binding sites; biogenesis; Biological Sciences; Biosynthesis; Chymotrypsin - metabolism; Electrophoretic Mobility Shift Assay; Gels; Genetic mutation; Immunoblotting; Models, Molecular; Molecular Sequence Data; Mutation; non-coding RNA; nucleases; Nucleic Acid Conformation; Plasmids; Proteases; Protein Binding; Protein Structure, Tertiary; proteinases; Proteins; ribonucleoproteins; ribosomal RNA; RNA; RNA precursors; RNA Precursors - genetics; RNA Precursors - metabolism; RNA, Fungal - genetics; RNA, Fungal - metabolism; RNA, Ribosomal - genetics; RNA, Ribosomal - metabolism; RNA, Small Nuclear - genetics; RNA, Small Nuclear - metabolism; RNA, Transfer - genetics; RNA, Transfer - metabolism; RNA, Untranslated - chemistry; RNA, Untranslated - genetics; RNA, Untranslated - metabolism; RNA-binding proteins; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sequence Homology, Amino Acid; Small nuclear RNA; stems; Transfer RNA; Trypsin - metabolism; Yeast; Yeasts

The La protein binds the 3' ends of many newly synthesized noncoding RNAs, protecting these RNAs from nucleases and influencing folding, maturation, and ribonucleoprotein assembly. Although 3' end binding by La involves the N-terminal La domain and adjacent RNA recognition motif (RRM), the...