Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

• Published in: Nature communications Vol. 8; no. 1; pp. 14902 - 10
• Main Authors: Xu, Shicai, Zhan, Jian, Man, Baoyuan, Jiang, Shouzhen, Yue, Weiwei, Gao, Shoubao, Guo, Chengang, Liu, Hanping, Li, Zhenhua, Wang, Jihua, Zhou, Yaoqi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.03.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 631/61/32; 639/301/357/918/1052; 639/638/11/511; 9/10; Biology; Biomarkers; Biosensing Techniques; Biosensors; Carbon; Cost-Benefit Analysis; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA Probes - chemistry; Fabrication; Genetic diversity; Graphene; Graphite - chemistry; High-Throughput Screening Assays - economics; High-Throughput Screening Assays - instrumentation; Humanities and Social Sciences; Hybridization; Kinetics; Limit of Detection; Miniaturization; Models, Chemical; multidisciplinary; Nanowires; Nucleic Acid Hybridization; Reproducibility of Results; Science; Sensors; Transistors; China

Reliable determination of binding kinetics and affinity of DNA hybridization and single-base mismatches plays an essential role in systems biology, personalized and precision medicine. The standard tools are optical-based sensors that are difficult to operate in low cost and to miniaturize for high-...