A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity

• Published in: Biotechnology letters Vol. 42; no. 5; pp. 773 - 785
• Main Authors: Larroude, Macarena, Trabelsi, Heykel, Nicaud, Jean-Marc, Rossignol, Tristan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2020; Springer Nature B.V; Springer Verlag
• Subjects: Applied Microbiology; Biochemistry; Biomarkers; Biomedical and Life Sciences; Biotechnology; CRISPR; CRISPR-Cas Systems; Experimentation; Fungal Proteins - genetics; gene editing; Gene Editing - methods; genes; genetic background; Genomes; gRNA; Homologous Recombination; Homology; Life Sciences; loci; Microbiology; Mutation; Nucleotide sequence; Original Research Paper; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - genetics; strain differences; Yarrowia - genetics; Yarrowia - growth & development; Yarrowia lipolytica; Yeast; yeasts; Synthetic biology; Golden Gate; CRISPR/Cas9; GSY1; Yarrowia lipolytica

Objectives The construction and validation of a set of Yarrowia lipolytica CRISPR/Cas9 vectors containing six different markers that allows virtually any genetic background to be edited, including those of wild-type strains. Results Using the Golden Gate method, we assembled a set of six CRISPR/Cas9...