Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

• Published in: Nature communications Vol. 8; no. 1; pp. 15939 - 8
• Main Authors: Tang, Weixin, Hu, Johnny H., Liu, David R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.06.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 45/41; 631/1647/1511; 631/61/201/2110; Active control; Canonical forms; Catalysis; CRISPR; CRISPR-Cas Systems; Editing; Gene Editing; Gene expression; Gene regulation; Genome editing; Genomes; HEK293 Cells; Humanities and Social Sciences; Humans; Ligands; Mammalian cells; multidisciplinary; Nuclease; Nucleic Acid Conformation; Nucleotide sequence; RNA, Catalytic - genetics; RNA, Catalytic - metabolism; RNA, Guide, CRISPR-Cas Systems - chemistry; RNA, Guide, CRISPR-Cas Systems - genetics; RNA, Guide, CRISPR-Cas Systems - metabolism; Science; Science (multidisciplinary); Transcription activation; Transcriptional Activation

Programmable sequence-specific genome editing agents such as CRISPR-Cas9 have greatly advanced our ability to manipulate the human genome. Although canonical forms of genome-editing agents and programmable transcriptional regulators are constitutively active, precise temporal and spatial control ove...