Intrauterine Zika virus infection of pregnant immunocompetent mice models transplacental transmission and adverse perinatal outcomes

• Published in: Nature communications Vol. 8; no. 1; p. 14575
• Main Authors: Vermillion, Meghan S., Lei, Jun, Shabi, Yahya, Baxter, Victoria K., Crilly, Nathan P., McLane, Michael, Griffin, Diane E., Pekosz, Andrew, Klein, Sabra L., Burd, Irina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.02.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/106; 13/51; 38; 38/77; 631/326/596/2555; 631/326/596/2557; 692/699/255/2514; 82; 82/80; Animal models; Animals; Animals, Newborn; Chlorocebus aethiops; Congenital diseases; Female; Fetuses; Gestational age; Humanities and Social Sciences; Humans; Immune system; Immunocompetence; Infections; Infectious Disease Transmission, Vertical; Medicine; Mice; Microcephaly; multidisciplinary; Pathogenesis; Placenta; Placenta - virology; Pregnancy; Pregnancy Complications, Infectious - virology; Pregnancy Outcome; Science; Science (multidisciplinary); Uterus - virology; Vagina; Vector-borne diseases; Vero Cells; Zika virus; Zika Virus - physiology; Zika Virus Infection - transmission; Zika Virus Infection - virology; United States > US; Maryland; Baltimore Maryland

Zika virus (ZIKV) crosses the placenta and causes congenital disease. Here we develop an animal model utilizing direct ZIKV inoculation into the uterine wall of pregnant, immunocompetent mice to evaluate transplacental transmission. Intrauterine inoculation at embryonic day (E) 10, but not E14, with...