Structural basis of human PCNA sliding on DNA

• Published in: Nature communications Vol. 8; no. 1; pp. 13935 - 7
• Main Authors: De March, Matteo, Merino, Nekane, Barrera-Vilarmau, Susana, Crehuet, Ramon, Onesti, Silvia, Blanco, Francisco J., De Biasio, Alfredo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10.01.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 101/6; 119/118; 140/131; 631/337/151/2353; 631/337/1644; 631/45/607/1163; 82/16; 82/80; 82/83; Crystal structure; Crystallography; Crystallography, X-Ray; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA Polymerase III - chemistry; DNA Polymerase III - metabolism; DNA Replication; DNA, B-Form - chemistry; DNA, B-Form - metabolism; Humanities and Social Sciences; Humans; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Dynamics Simulation; multidisciplinary; Mutation; Phosphates; Proliferating Cell Nuclear Antigen - chemistry; Proliferating Cell Nuclear Antigen - genetics; Proliferating Cell Nuclear Antigen - metabolism; Science; Science (multidisciplinary); Spain

Sliding clamps encircle DNA and tether polymerases and other factors to the genomic template. However, the molecular mechanism of clamp sliding on DNA is unknown. Using crystallography, NMR and molecular dynamics simulations, here we show that the human clamp PCNA recognizes DNA through a double pat...