Priming of neutrophil oxidative burst in diabetes requires preassembly of the NADPH oxidase

• Published in: Journal of leukocyte biology Vol. 84; no. 1; pp. 292 - 301
• Main Authors: Omori, Kazuhiro, Ohira, Taisuke, Uchida, Yushi, Ayilavarapu, Srinivas, Batista, Eraldo L., Yagi, Motohiko, Iwata, Tomoyuki, Liu, Hongsheng, Hasturk, Hatice, Kantarci, Alpdogan, Van Dyke, Thomas E.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.07.2008; The Society for Leukocyte Biology
• Subjects: Adult; cell activation; Cell Membrane - drug effects; Cell Membrane - enzymology; Diabetes Mellitus - enzymology; Diabetes Mellitus - pathology; Female; Flavonoids - pharmacology; HL-60 Cells; Humans; inflammation; Male; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - metabolism; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; NADPH Oxidases - metabolism; Neutrophils - drug effects; Neutrophils - enzymology; Neutrophils - pathology; Oxidative Stress; p38 Mitogen-Activated Protein Kinases - metabolism; Phosphorylation - drug effects; Protein Transport - drug effects; Receptor for Advanced Glycation End Products; Receptors, Immunologic - metabolism; Receptors, Signal Transduction, and Genes; Respiratory Burst - drug effects; signal transduction; Superoxides - metabolism

Hyperglycemia associated with diabetes mellitus results in the priming of neutrophils leading to oxidative stress that is, in part, responsible for diabetic complications. p47phox, a NADPH oxidase cytosolic subunit, is a key protein in the assembly of the NADPH oxidase leading to superoxide generati...