金属离子与氨羧类螯合配体的键合特性研究
利用前沿色谱法,通过Cu2＋、Ni2＋和Co2＋与螯合配体IDA键合的准确度（R2〉0.98）与精密度（RSD〈5%）实验,研究了前沿色谱法同时测定络合稳定常数KML与总键合位点数Λ0值的可行性。为了进一步证明前沿色谱法的普适性,以Cu2＋、Ni2＋和Co2＋为代表,在3种键合缓冲体系（Na Ac-HAc、Na-PB、Tris-HCl）中,考察了金属离子在3种氨羧类螯合配体（IDA、Asp、Glu）上络合稳定常数KML的变化规律。结果表明,螯合配体对金属离子键合强度遵循IDA〉Asp〉Glu;金属离子对螯合配体键合强度遵循Cu2＋〉Ni2＋〉Co2＋;3种键合缓冲体系中,Na Ac-HAc键合...
Saved in:
| Published in | 分析化学 Vol. 45; no. 9; pp. 1303 - 1308 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
西北大学化工学院,西安,710069
2017
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.11895/j.issn.0253-3820.170154 |
Cover
| Abstract | 利用前沿色谱法,通过Cu2＋、Ni2＋和Co2＋与螯合配体IDA键合的准确度（R2〉0.98）与精密度（RSD〈5%）实验,研究了前沿色谱法同时测定络合稳定常数KML与总键合位点数Λ0值的可行性。为了进一步证明前沿色谱法的普适性,以Cu2＋、Ni2＋和Co2＋为代表,在3种键合缓冲体系（Na Ac-HAc、Na-PB、Tris-HCl）中,考察了金属离子在3种氨羧类螯合配体（IDA、Asp、Glu）上络合稳定常数KML的变化规律。结果表明,螯合配体对金属离子键合强度遵循IDA〉Asp〉Glu;金属离子对螯合配体键合强度遵循Cu2＋〉Ni2＋〉Co2＋;3种键合缓冲体系中,Na Ac-HAc键合效果最好。按照M06/6-311＋＋G（d,p）方法对螯合配体与金属离子的结合能（ΔE）与吉布斯自由能（ΔG）进行相关的量子计算。根据ΔE与ΔG的大小,从理论上推测出螯合配体与金属离子的键合规律,此规律与上述实验结果基本相符。本研究为金属离子与螯合配体间键合参数的求取提供了有效的方法和手段,从而为今后提高IMAC柱的稳定性,解决固定金属亲和柱在应用过程中尤其是竞争洗脱过程中金属离子的流失问题奠定了良好的基础。 |
|---|---|
| AbstractList | 利用前沿色谱法,通过Cu2+、Ni2+和Co2+与螯合配体IDA 键合的准确度(R2>0.98)与精密度(RSD <5%)实验,研究了前沿色谱法同时测定络合稳定常数KML与总键合位点数Λ0值的可行性.为了进一步证明前沿色谱法的普适性,以Cu2+、Ni2+和Co2+为代表,在3种键合缓冲体系(NaAc-HAc、Na-PB、Tris-HCl)中,考察了金属离子在3种氨羧类螯合配体(IDA、Asp、Glu)上络合稳定常数KML的变化规律.结果表明,螯合配体对金属离子键合强度遵循IDA>Asp>Glu;金属离子对螯合配体键合强度遵循Cu2+>Ni2+>Co2+;3种键合缓冲体系中,NaAc-HAc键合效果最好.按照M06/6-311++G (d, p) 方法对螯合配体与金属离子的结合能(ΔE)与吉布斯自由能(ΔG)进行相关的量子计算.根据ΔE 与ΔG 的大小,从理论上推测出螯合配体与金属离子的键合规律,此规律与上述实验结果基本相符.本研究为金属离子与螯合配体间键合参数的求取提供了有效的方法和手段,从而为今后提高IMAC柱的稳定性,解决固定金属亲和柱在应用过程中尤其是竞争洗脱过程中金属离子的流失问题奠定了良好的基础. 利用前沿色谱法,通过Cu2＋、Ni2＋和Co2＋与螯合配体IDA键合的准确度（R2〉0.98）与精密度（RSD〈5%）实验,研究了前沿色谱法同时测定络合稳定常数KML与总键合位点数Λ0值的可行性。为了进一步证明前沿色谱法的普适性,以Cu2＋、Ni2＋和Co2＋为代表,在3种键合缓冲体系（Na Ac-HAc、Na-PB、Tris-HCl）中,考察了金属离子在3种氨羧类螯合配体（IDA、Asp、Glu）上络合稳定常数KML的变化规律。结果表明,螯合配体对金属离子键合强度遵循IDA〉Asp〉Glu;金属离子对螯合配体键合强度遵循Cu2＋〉Ni2＋〉Co2＋;3种键合缓冲体系中,Na Ac-HAc键合效果最好。按照M06/6-311＋＋G（d,p）方法对螯合配体与金属离子的结合能（ΔE）与吉布斯自由能（ΔG）进行相关的量子计算。根据ΔE与ΔG的大小,从理论上推测出螯合配体与金属离子的键合规律,此规律与上述实验结果基本相符。本研究为金属离子与螯合配体间键合参数的求取提供了有效的方法和手段,从而为今后提高IMAC柱的稳定性,解决固定金属亲和柱在应用过程中尤其是竞争洗脱过程中金属离子的流失问题奠定了良好的基础。 |
| Abstract_FL | The feasibility of frontal chromatography for determining the complexation stability constant KML and total mole of binding site Λo was demonstrated by the accuracy and precision binding experiments between metal ions (Cu2+, Ni2+ and Co2+) and chelating ligand (IDA), in which R2>0.98 and RSD <5%.To further prove the universality of the frontal chromatography, the changing rules of KML values between Cu2+, Ni2+, Co2+ and IDA, Asp, L-Glu were examined under NaAc-HAc, Na-PB and Tris-HCl buffer systems.The results showed that the binding strength of chelating ligands for metal ions followed IDA>Asp>Glu;binding strength of metal ions for chelate ligands followed Cu2+>Ni2+>Co2+;and the binding effect with NaAc-HAc buffer was the best.In aqueous solution, quantum computing of binding energy (ΔE) and gibbs free energy (ΔG) between chelating ligand and metal ion was performed at the M06/6-311++G (d, p) level.According to ΔE and ΔG, the binding rules between chelating ligand and metal ion were predicted theoretically.These rules were basically in agreement with above experimental results.The present work provided effective method for studying on binding characteristics of metal ions for aminocarboxyl chelating ligands, thus exhibited a good foundation for improving the stability of immobilized metal affinity chromatographic column and solving the leakage of metal ions from the column in the process of competitive elution. |
| Author | 陈斌 王石慧 李蓉 樊安 |
| AuthorAffiliation | 西北大学化工学院,西安710069 |
| AuthorAffiliation_xml | – name: 西北大学化工学院,西安,710069 |
| Author_FL | FAN An LI Rong CHEN Bin WANG Shi-Hui |
| Author_FL_xml | – sequence: 1 fullname: CHEN Bin – sequence: 2 fullname: WANG Shi-Hui – sequence: 3 fullname: LI Rong – sequence: 4 fullname: FAN An |
| Author_xml | – sequence: 1 fullname: 陈斌 王石慧 李蓉 樊安 |
| BookMark | eNo9j7tKA0EYhaeIYIx5BxEsN_6zs7P7TynBGwRs0ofZyyQbdKJZxNhZKGqhRExlEayMFl7SiBL0abIZ8xYuRKwO5_BxDmeB5HRLR4QsUShRioKvNktxkugS2JxZDO0s9oByJ0fy_9E8KSZJ7ANQCg5yzBMxPb9Jh33zMEqfu-OP68nbo_kemOHop_-adi-mZ1fjr1tzdzrtvWTWXH5OTgbmvmee3hfJnJK7SVT80wKpbqxXy1tWZWdzu7xWsQLuoeWKKAQAN5AeZ0qwKLAdnyK6kS8l-DxEkCqMfKSMI3VdhwkFwgtCiSqwQ58VyMqs9khqJXW91mwdtnU2WFOdRseG7KQAwIxbnnFBo6XrB3FG7rfjPdk-rrkeA4G2Q9kv5uxrbg |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.11895/j.issn.0253-3820.170154 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| DocumentTitleAlternate | Study on Binding Characteristics of Aminocarboxyl Chelating Ligands for Metal Ions |
| DocumentTitle_FL | Study on Binding Characteristics of Aminocarboxyl Chelating Ligands for Metal Ions |
| EndPage | 1308 |
| ExternalDocumentID | fxhx201709008 673098241 |
| GrantInformation_xml | – fundername: 国家自然科学基金; 陕西省自然科学基金; 陕西省教育厅服务地方专项; 陕西省产业化项目(No.2013jc23)资助.This work was supported by the National Natural Science Foundation of China; the Natural Science Foundation of Shaanxi Province, China; the Service Local Special Project of Shaanxi Province Education Department; the Industrialization Cultivation Item of Shaanxi Province Educational Department funderid: (21376191); (2011JM2013); (14JF027); (21376191); (2011JM2013); (14JF027); (2013jc23) |
| GroupedDBID | --K --M .~1 0R~ 1B1 1~. 1~5 29B 2B. 2C. 2RA 4.4 457 4G. 5GY 5VS 7-5 71M 8P~ 92E 92I 92L AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AARLI AAXUO ABFNM ABMAC ABXDB ABYKQ ACDAQ ACGFS ACNNM ACRLP ADBBV ADECG ADEZE ADMUD AEBSH AEKER AENEX AFKWA AFTJW AFZHZ AGHFR AGUBO AGYEJ AIEXJ AIKHN AITUG AJBFU AJOXV AJSZI ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC CQIGP CS3 CW9 DU5 EBS EFJIC EFLBG EJD EO9 EP2 EP3 FDB FEDTE FIRID FLBIZ FNPLU FYGXN GBLVA HVGLF HZ~ J1W KOM M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 ROL SDF SDG SES SPC SPCBC SSK SSZ T5K TCJ TGP U1G U5L ~G- ~WA -02 -0B -SB -S~ .5D 188 4A8 5VR 5XA 5XC 8RM 92M 93N 9D9 9DB AAITT AATTM AAXKI AAYWO ABJNI ABWVN ACRPL ADNMO ADVLN AEIPS AFJKZ AFUIB AFXIZ AGCQF AGRNS AIIUN AKRWK ANKPU APXCP BBR BNPGV CAJEB CCEZO CDRFL CDYEO CJPJV CW7 FA0 J.9 JUIAU M~T PB1 PB6 PSX Q-- R-B RT2 S.. SSH T8R U1F U5B UY8 UZ4 W94 ~LN |
| ID | FETCH-LOGICAL-c578-69ed0006ca753f93ec24b1886ebaa0b5d80afdeb81358166439f097cda8fc2db3 |
| ISSN | 0253-3820 |
| IngestDate | Thu May 29 04:00:23 EDT 2025 Wed Feb 14 09:58:08 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Keywords | 氨羧类螯合配体 Complexation stability constant Frontal chromatography Quantum computing Aminocarboxyl chelating ligand 络合稳定常数 总键合位点数 量子计算 前沿色谱法 Total mole of binding site |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c578-69ed0006ca753f93ec24b1886ebaa0b5d80afdeb81358166439f097cda8fc2db3 |
| Notes | CHEN Bin, WANG Shi-Hui, LI Rong, FAN An （ School of Chemical Engineering, Northwest University, Xi＇an 710069, China） 22-1125/O6 The feasibility of frontal chromatography for determining the complexation stability constant KMLand total mole of binding site Λowas demonstrated by the accuracy and precision binding experiments between metal ions（ Cu2＋,Ni2＋and Co2＋） and chelating ligand（ IDA）,in which R2〉0. 98 and RSD 〈5%. To further prove the universality of the frontal chromatography,the changing rules of KMLvalues between Cu2＋,Ni2＋,Co2＋and IDA,Asp,L-Glu were examined under Na Ac-HAc,Na-PB and Tris-HCl buffer systems. The results showed that the binding strength of chelating ligands for metal ions followed IDA 〉Asp 〉Glu; binding strength of metal ions for chelate ligands followed Cu2＋〉Ni2＋〉Co2＋; and the binding effect with Na Ac-HAc buffer was the best. In aqueous solution,quantum computing of binding energy（ ΔE） and gibbs free energy（ ΔG）between chelating ligand and metal ion was performed at the M06/6-311 ＋＋G（ d |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_fxhx201709008 chongqing_primary_673098241 |
| PublicationCentury | 2000 |
| PublicationDate | 2017 |
| PublicationDateYYYYMMDD | 2017-01-01 |
| PublicationDate_xml | – year: 2017 text: 2017 |
| PublicationDecade | 2010 |
| PublicationTitle | 分析化学 |
| PublicationTitleAlternate | Chinese Journal of Analytical Chemistry |
| PublicationTitle_FL | Chinese Journal of Analytical Chemistry |
| PublicationYear | 2017 |
| Publisher | 西北大学化工学院,西安,710069 |
| Publisher_xml | – name: 西北大学化工学院,西安,710069 |
| SSID | ssib001104858 ssib048394981 ssj0037577 ssib002258198 ssib007686865 ssib051370160 |
| Score | 2.1141186 |
| Snippet | 利用前沿色谱法,通过Cu2＋、Ni2＋和Co2＋与螯合配体IDA键合的准确度（R2〉0.98）与精密度（RSD〈5%）实验,研究了前沿色谱法同时测定络合稳定常数KML与总键合位点数Λ0值的可行性。为了进一步证明前沿色谱法的普适性,以Cu2＋、Ni2＋和Co2＋为代表,在3种键合缓冲体系（Na... 利用前沿色谱法,通过Cu2+、Ni2+和Co2+与螯合配体IDA 键合的准确度(R2>0.98)与精密度(RSD... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 1303 |
| SubjectTerms | 前沿色谱法 总键合位点数 氨羧类螯合配体 络合稳定常数 量子计算 |
| Title | 金属离子与氨羧类螯合配体的键合特性研究 |
| URI | http://lib.cqvip.com/qk/93919X/201709/673098241.html https://d.wanfangdata.com.cn/periodical/fxhx201709008 |
| Volume | 45 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR27btRAcBWFAhrEU4RAlIKtIh_r1-5saV8cRUhQBSndyeuzc9WFx0WKUiAKEFCAgkhFEVERKAikQaAIviaJub9gZs93Z6GAgMZaz87sjD3rmdn17ixj13RocGySKUcFGThBUSgnLQR-V55G5-V6RqU0ULx5Sy7eDm4sh8sTE7u1VUtrPdPINo7dV_I_WkUY6pV2yf6DZkeNIgDLqF-8oobx-lc65onmOJjXLk9CHrtcJzxRPJI8jgkSzXMteBLwGDhgFcIFj4Bw4oRHyhZciwxEGy0QFZIA2JZDDvOWHNvxCVlHHAKq0gGPkhqy4qB5rIkFiKrlSBAaFbBK1oNgIkQqkISPfElIhDS5lpXY0Wi-0HLTlomkemjOWXYJh9iKtMBj3_JFQjVnsebt0wIJDdqC8KEhsm0jna7PdAy2dFam0At9xwdP1O32IA1l1T91zQiTW645dLyF450F6NB6C-LRGPFoUIr6QWbrX1JxSzSHGjzKk3DCU8qlJaSNB_X0eWgPa3-Z0VZiwDW-x6GdhPFf5QCD00CP9_2Grq8o4d8wkvBVaE8OHUk2XImGcl__jdSUIqSz2l25i4GP3YfWLdLuSi1kWjrDTldjndlo0HHPsomNzjl2sjk8YvA80_0nLw_3tsu3-4cfNg--vDj69K78vlPu7f_Y_ni4-bT_-PnBt1fl60f9rV28LZ99PXq4U77ZKt9_vsCWFpKl5qJTneXhZOgTHKnzNkVGWYrD40L7eeYFxgWQuUlTYcI2iLRo5wZcyscnKUwuhFZZO4Ui89rGv8gmu6vd_BKbLSA3MpCpbBsTiFQbT6SZLwpXqsy4qT_FpkdvoHVnkLKlNdLcFJup3kmr-pDvt4r1zjr1N6ExIL78R_JpdoowB3NwV9hk795afhWj0p6ZsV3hJ3X7bUI |
| linkProvider | Elsevier |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E9%87%91%E5%B1%9E%E7%A6%BB%E5%AD%90%E4%B8%8E%E6%B0%A8%E7%BE%A7%E7%B1%BB%E8%9E%AF%E5%90%88%E9%85%8D%E4%BD%93%E7%9A%84%E9%94%AE%E5%90%88%E7%89%B9%E6%80%A7%E7%A0%94%E7%A9%B6&rft.jtitle=%E5%88%86%E6%9E%90%E5%8C%96%E5%AD%A6&rft.au=%E9%99%88%E6%96%8C+%E7%8E%8B%E7%9F%B3%E6%85%A7+%E6%9D%8E%E8%93%89+%E6%A8%8A%E5%AE%89&rft.date=2017&rft.issn=0253-3820&rft.volume=45&rft.issue=9&rft.spage=1303&rft.epage=1308&rft_id=info:doi/10.11895%2Fj.issn.0253-3820.170154&rft.externalDocID=673098241 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F93919X%2F93919X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Ffxhx%2Ffxhx.jpg |