Differential effects of monocular deprivation on glutamic acid decarboxylase and type II calcium-calmodulin-dependent protein kinase gene expression in the adult monkey visual cortex [published erratum appears in J Neurosci 1991 May;11(5):following Table of Contents]

• Published in: The Journal of neuroscience Vol. 11; no. 1; pp. 31 - 47
• Main Authors: Benson, DL, Isackson, PJ, Gall, CM, Jones, EG
• Format: Journal Article
• Language: English
• Published: Washington, DC Soc Neuroscience 01.01.1991; Society for Neuroscience
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Calcium-Calmodulin-Dependent Protein Kinases; Cloning, Molecular; Eye and associated structures. Visual pathways and centers. Vision; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Glutamate Decarboxylase - genetics; Macaca; Macaca fascicularis; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Protein Kinases - genetics; Transcription, Genetic; Vertebrates: nervous system and sense organs; Vision, Monocular; Visual Cortex - enzymology; Visual Cortex - physiology; Brain; Molecular hybridization; Visual cortex; Nucleotide sequence; Monkey; Central nervous system; Monocular vision; Glutamate decarboxylase; Gene expression; Vertebrata; Mammalia; Messenger RNA; Visual pathway; Protein kinase; Topography; Primates
• DOI: 10.1523/jneurosci.11-01-00031.1991
• ISSN: 0270-6474

Increases in immunocytochemically detectable type II calcium-calmodulin-dependent protein kinase (CaM II kinase) and decreases in immunocytochemically detectable glutamic acid decarboxylase (GAD) are known to occur in the visual cortex of adult monkeys following brief periods of monocular visual deprivation. In the present study, GAD and CaM II kinase gene expression was investigated under these conditions. The polymerase chain reaction (PCR) was used to generate species-specific cDNA clones that were used to make antisense RNA probes. A second form of CaM II kinase alpha, CaM II kinase alpha-33, which contains an additional phosphorylation consensus sequence, was identified. In situ hybridization in normal visual cortex revealed a complex sublaminar organization of GAD-expressing cells within layers IVC and VI and a distribution of CaM II kinase alpha-expressing cells that was greatest in layers II, III, IVB, and VI. In situ hybridization in the cortex from animals that had been monocularly deprived revealed enhanced CaM II kinase mRNA levels in deprived-eye columns of layer IVC and, associated with the deprived eye, cytochrome oxidase-stained periodicities in other layers. In layer IV, the enhancement of labeling in deprived-eye stripes was, on average, 16% greater than in normal-eye stripes. By contrast, GAD, mRNA levels appeared unchanged in all layers, suggesting a posttranscriptional regulatory mechanism.