Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract

• Published in: Scientific reports Vol. 8; no. 1; pp. 853 - 14
• Main Authors: Cozens, Daniel, Grahame, Edward, Sutherland, Erin, Taylor, Geraldine, Berry, Catherine C., Davies, Robert L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.01.2018; Nature Publishing Group
• Subjects: 13/106; 13/107; 14/1; 14/19; 14/28; 14/63; 631/1647/767/1424; 631/1647/767/2201; Animals; Bronchi - cytology; Cattle; Cell culture; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Economic importance; Epidermal growth factor; Epidermal Growth Factor - pharmacology; Epithelial cells; Epithelial Cells - cytology; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelium; Humanities and Social Sciences; multidisciplinary; Oxygen - metabolism; Oxygen tension; Respiratory tract; Respiratory tract diseases; Retinoic acid; Science; Science (multidisciplinary); Serum-free medium; Thyroid hormones; Tretinoin - pharmacology; Triiodothyronine; Vaccines; Viral infections

Cattle are subject to economically-important respiratory tract infections by various bacterial and viral pathogens and there is an urgent need for the development of more realistic in vitro models of the bovine respiratory tract to improve our knowledge of disease pathogenesis. In the present study,...