Formulation, characterization and evaluation of mRNA-loaded dissolvable polymeric microneedles (RNApatch)

• Published in: Scientific reports Vol. 8; no. 1; pp. 11842 - 11
• Main Authors: Koh, Kai Jun, Liu, Yi, Lim, Seng Han, Loh, Xian Jun, Kang, Lifeng, Lim, Chee Yen, Phua, Kyle K. L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.08.2018; Nature Publishing Group
• Subjects: 42/109; 59/5; 631/1647/2300; 631/1647/2300/1851; Efficiency; Fabrication; Gene expression; Genetic engineering; Humanities and Social Sciences; Immune response (humoral); Immunotherapy; Injection; Mechanical properties; Molecular weight; mRNA; multidisciplinary; Nanoparticles; Pharmacy; Polymers; Polyvinylpyrrolidone; Purification; Science; Science (multidisciplinary); Transfection

In this paper, we report a proof of concept study on the fabrication, characterization and therapeutic evaluation of in vitro transcribed messenger RNA (mRNA) loaded in a dissolving microneedle patch (RNApatch). We show that low molecular weight polyvinylpyrrolidone (PVP) can directly be used withou...