Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system

• Published in: Scientific reports Vol. 9; no. 1; p. 671
• Main Authors: Murakami, Satoshi, Matsumoto, Rena, Kanamori, Takashi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.01.2019; Nature Publishing Group
• Subjects: 631/337/470; 631/337/574; 82/1; 82/80; Cell culture; Cofactors; DNA biosynthesis; DnaK protein; ErbB-2 protein; High-throughput screening; Humanities and Social Sciences; Immunoglobulin G; Monoclonal antibodies; multidisciplinary; Protein biosynthesis; Protein synthesis; Proteins; Science; Science (multidisciplinary); Trastuzumab

IgG is an indispensable biological experimental tool as well as a widely-used therapeutic protein. However, cell culture-based expression of monoclonal IgG is costly and time-consuming, making this process difficult to use for high-throughput screening in early-stage evaluation of biologics. With th...