Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase - Sakurai, Takayuki; Kamiyoshi, Akiko; Takei, Norio; Watanabe, Satoshi; Sato, Masahiro; Shindo, Takayuki (Scientific reports, 2019)
Keywords: 101/47; 38/70; 38/77; 631/1647/1511; 631/1647/277; 64/60; 82/29; Alleles; Animals; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; DNA; DNA-directed DNA polymerase; Embryos; Female; Gene Editing; Genomes; High-throughput screening; Humanities and Social Sciences; Magnesium chloride; Male; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; multidisciplinary; Mutants; Mutation; Nucleotide sequence; Nucleotides; Polymerase chain reaction; Polymerase Chain Reaction - methods; Receptor activity modifying proteins; Receptor Activity-Modifying Protein 1 - antagonists & inhibitors; Receptor Activity-Modifying Protein 1 - genetics; Receptor Activity-Modifying Protein 3 - antagonists & inhibitors; Receptor Activity-Modifying Protein 3 - genetics; Ribonucleic acid; RNA; RNA, Untranslated - antagonists & inhibitors; RNA, Untranslated - genetics; Science; Science (multidisciplinary); Spacer; Taq Polymerase - genetics; Taq Polymerase - metabolism; Thermus - enzymology