Optimization for biogenic microbial synthesis of silver nanoparticles through response surface methodology, characterization, their antimicrobial, antioxidant, and catalytic potential

• Published in: Scientific reports Vol. 11; no. 1; pp. 770 - 18
• Main Authors: Ibrahim, Saba, Ahmad, Zahoor, Manzoor, Muhammad Zeeshan, Mujahid, Muhammad, Faheem, Zahra, Adnan, Ahmad
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.01.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 639/638; 639/638/224; 639/638/45; 639/638/60; 639/638/77; 639/925; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Antioxidants; Antioxidants - pharmacology; Bacillus cereus; Bacillus cereus - drug effects; Bacillus cereus - growth & development; Bacillus cereus - metabolism; Catalysis; E coli; Fourier transforms; Heavy metals; Humanities and Social Sciences; Inoculum; Metal Nanoparticles - chemistry; multidisciplinary; Multidrug resistance; Nanoparticles; Particle Size; pH effects; Scanning electron microscopy; Science; Science (multidisciplinary); Silver; Silver - chemistry; Silver - metabolism; Soil contamination; Soil pollution; Soil temperature; Spectrometry; Sulfonic acid; X-Ray Diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-80805-0

Silver is a poisonous but precious heavy metal that has widespread application in various biomedical and environmental divisions. Wide-ranging usage of the metal has twisted severe environmental apprehensions. Henceforth there is a cumulative call for the progress of modest, low-cost and, the ecological method for remediation of silver. In the present study, Bacillus cereus was isolated from contaminated soil. Various experimental factors like the amount of AgNO 3 , inoculum size, temperature, time, and pH were improved by using central composite design (CCD) grounded on response surface methodology (RSM). Optimized values for AgNO 3 (1 mM) 10 ml, inoculum size ( Bacillus cereus ) 8.7 ml, temperature 48.5 °C, time 69 h, and pH 9 showed in the form of optimized ramps. The formed nanoparticles stayed characterized by UV–visible spectrophotometer, Scanning Electron Microscopy, Fourier transform infra-red spectrometry, particle size analyzer, and X-ray diffraction. The particle size ranges from 5 to 7.06 nm with spherical form. The antimicrobial effectiveness of synthesized nanoparticles was tested contrary to five multidrug resistant microbial strains, Staphylococcus epidermidis, Staphylococcus aureus , Escherichia coli, Salmonella enterica, Porteus mirabilis by disc diffusion method. The minimum inhibitory concentrations and minimum lethal concentrations were detected by the broth macro dilution method. 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH) was used to check the free radical scavenging ability of biogenic silver nanoparticles. Similarly, anti-radical activity was checked by 2,2′-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS) with varying time intervals. Catalytic potential of biosynthesized silver nanoparticles was also investigated.